Ku70与hTERT在胃癌组织中的表达及其意义

目的探讨Ku70和人端粒酶逆转录酶(h TERT)蛋白表达与胃癌发生发展的关系及两者之间的联系。方法应用免疫组织化学方法检测Ku70和h TERT蛋白在10例正常胃黏膜、15例胃癌前病变黏膜和20例胃癌组织中的表达。结果 Ku70蛋白阳性表达定位于正常胃黏膜和胃癌组织的细胞核,胃癌前病变黏膜的细胞浆,其阳性表达率分别为90%、100%和80%;h TERT蛋白在正常胃黏膜几乎不表达,在胃癌前病变黏膜和胃癌组织中均定位于细胞浆,其阳性表达率分别为30%、100%和100%。胃癌组织中Ku70和h TERT蛋白的表达强度明显高于正常胃黏膜和胃癌前病变黏膜。Ku70和h TERT蛋白的表达显著相关。结论 Ku70和h TERT蛋白可能与胃癌的发生发展过程相关。

Expression of Ku70 and hTERT in Human Gastric Cancer and the Clinical Significance

Objective To explore the relationship of Ku70 and hTERT protein expressions in the progression of human gastric carcinogenesis and their correlations. Methods Expressions of Ku70 and hTERT protein in 10 normal gastric mucosa,15 precancerous lesions and 20 gastric cancers were examined by immunohistochemistry. Results Ku70 was found in normal gastric mucosa with mild nuclear expression,in precancerous lesion with mild cytoplasmic expression and in GC with marked nuclear expression and its positive expression rates were 90%,80% and 100% respectively.hTERT was found in cytoplasm and its positive expression rates in normal gastric mucosa,precancerous lesion and gastric cancer were respectively 20%,100% and 100%.The staining densities of Ku70 and hTERT protein in gastric cancer tissues were significantly higher than that in normal gastric mucosa and precancerous lesions.There was a significant correlation between the expressions of Ku70 and hTERT. Conclusion Ku70 and hTERT protein protein might play an important role in the progression of human gastric carcinogenesis.