血管内皮生长因子-C及p63在早期食管癌和上皮瘤变组织中的表达

目的探讨血管内皮生长因子-C（VEGF—C）和p63蛋白在早期食管癌及上皮内瘤变组织中的表达及其临床意义。方法随机选取正常食管黏膜、低级别上皮内瘤变、高级别上皮内瘤变及早期食管癌组织共146例，应用免疫组织化学EnVision二步法检测VEGF—C和p63蛋白在不同病理阶段中的表达。结果不同病理阶段的VEGF—C表达差异有统计学意义（X^2=47．455，P〈0．001）。正常黏膜与高级别上皮内瘤变（X^2=26．483，P〈0．001）、正常黏膜与早期癌（X^2=36．721，P〈0．001）、低级别上皮内瘤变与高级别上皮内瘤变（X^2=10．025，P〈0．0083）、低级别上皮内瘤变与早期癌（X^2=16．734，P〈0．001）之间VEGF-C的表达量构成比差异均有统计学意义。病理阶段与VEGF-C表达程度间呈正相关（r=0．462，P〈0．001）。不同病理阶段的p63表达量构成比差异有统计学意义（X^2=28．962，P〈0．05）。正常黏膜分别与低级别上皮内瘤变（X^2=12．735，P=0．005）、高级别上皮内瘤变（X^2=20．421，P〈0．001）、早期癌（X^2=20．854，P〈0．001）各组间的p63表达量构成比差异均有统计学意义；病理阶段与p63表达程度间呈正相关（r=0．272，P〈0．05）。结论VEGF—C和p63蛋白与早期食管癌和上皮内瘤变发生显著相关，可作为预警指标。

Expression of VEGF -C and p63 in early esophageal carcinoma and intraepithelial neoplasia

Objective To explore expression and clinical value of VEGF-C and p63 in early esophageal carcinoma and intraepithelial neoplasia. Methods 146 cases were randomized into normal esophageal mucosa, low level intraepithelial tumor, high level intraepithelial tumor and early esophageal carcinoma. The expression of VEGF-C and p63 were detected by using the immunohistochemistry dyeing.Results The expression of VEGF-C immunohistochemistry dyeing had statistical differences among different levels(X~2= 47.455, P <0.001). Normal esophageal mucosa v.s. high level intraepithelial tumor (X~2=36.721, P <0.001), Normal esophageal mucosa v.s. early esophageal carcinoma (X~2=26.483, P <0.001), low level intraepithelial tumor v.s. high level intraepithelial tumor(X~2= 10.025, P<0.0083), low level intraepithelial tumor v.s. early esophageal carcinoma(X~2=16.734, P<0.001). There was a significant correlation between pathological classification and the expression amount of VEGF-C (r = 0.462, P <0.001). The expression of p63 had statistical differences among different levels(X~2=28.962, P <0.05). There was a significant difference on normal esophageal mucosa comparing with low level, high level intraepithelial tumor or early esophageal carcinoma (X~2=12.735, P =0.005, X~2=20.421, P<0.001, X~2=20.854, P<0.001). There was a significant correlation between pathological classification and the expression of p63 (r= 0.272, P<0.05). Conclusion There is a significant correlation in the express of either VEGF-C or p63 comparing with either intraepithelial tumor or early esophageal carcinoma. It may be an early warning indicator.