新城疫病毒诱导肿瘤细胞凋亡的实验研究

目的：研究新城疫病毒Lasota株（NDV－L）杀伤肿瘤细胞及诱导肿瘤细胞凋亡的作用。方法：通过噬斑形成单位、HA效价、MTT法等方法检测NDV－L对细胞的杀伤作用；通过Giemsa's染色法、倒置相差显微镜、透射电镜、DNA凝胶电泳、流式细胞仪检测NDV－L诱导肿瘤细胞凋亡的作用。结果：NDV对肿瘤细胞有选择性杀伤作用。经NDV－L诱导后，肿瘤细胞呈现典型的凋亡特征。结论：NDV除通过自身复制直接杀伤肿瘤细胞外，还具有诱导肿瘤细胞凋亡的作用。

EXPERIMENTAL STUDY ON INDUCING EFFECT OF NEWCASTLE DECEASE VIRUS ON APOPTOSIS OF CANCER CELLS

OBJECTIVE To study the killing and inducing effects of Lasota strain of Newcastle decease virus (NDV-L) on cancer cells.METHODS The killing effects of NDV-L on cancer cells and normal cell were observed by means of plaque assays, HA titer test and MTT test. The inducing effects of NDV-L on apoptosis were observed by means of Giemsa's dying test, phase contrast light microscopy, transmission electron microscope, DNA extraction, and flow cytometry.RESULTS NDV-L selectively replicated in and kills cancer cells. After being induced by NDV-L, caner cells showed some typical signs of cells undergoing apoptosis.CONCLUSION NDV-L kills cancer cells not only by directly replicating in them but also by causing them apoptosis.