| 沙门菌质粒DNA上整合子的分析 |
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| 引用本文： | 蔡俊源,杨荣兴. 沙门菌质粒DNA上整合子的分析[J]. 中国热带医学, 2009, 9(8): 1401-1401,1595 |
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| 作者姓名： | 蔡俊源 杨荣兴 |
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| 作者单位： | 1. 深圳市盐田区疾病预防控制中心,广东,深圳,518081
2. 深圳市疾病预防控制中心,广东,深圳,518000 |
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| 摘 要： | 目的分析沙门菌质粒DNA上整合子的分子特征。方法用全自动细菌分析仪Mieroscan WalkAway40和纸片扩散法，对32株沙门菌进行抗生素敏感性测定，提取细菌质粒DNA，PCR扩增Ⅰ类整合子、Ⅱ类和Ⅲ类整合酶，对扩增产物进行序列分析。结果12株细菌含有一个Ⅰ类整合子，整合子大小分别为1000bp和1700bp。1000bp整合子含基因盒aadAl，1700bp整合子含基因盒dfr17-aadA5，未检测到Ⅱ类和Ⅲ类整合酶。结论整合子在沙门菌中广泛存在，整合子是介导和传播细菌耐药性的重要分子机制。
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| 关 键 词： | 沙门菌 整合子 基因盒 耐药 |
Investigation into the itegrons on plasmid DNA of Salmonella |
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| CAI Jun-yuan,YANG Rong-xing. Investigation into the itegrons on plasmid DNA of Salmonella[J]. China Tropical Medicine, 2009, 9(8): 1401-1401,1595 |
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| Authors: | CAI Jun-yuan YANG Rong-xing |
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| Affiliation: | . (Yantian District Center for Disease Control and Prevention, Shenzhen 518081, Guangdong, P. R.China ) |
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| Abstract: | Objective To analyze the molecular feature of integrons on plasmid DNA in Salmonella. Methods The susceptibility of 32 Salmonella isolates to antibiotics were tested by Microscan WalkAway40 and the disk diffusion assay. Plasmid DNA was extracted. Class 1 integrons,intI2 and intI3 were detected by PCR. The purified amplicons of class 1 integrons were sequenced,and gene cassettes were identified. Results There 12 isolates carried class 1 integrons on plasmid DNA. The sizes of class 1 integrons were 1 000bp and 1 700bp respectively. 1 000bp integron carried gene cassette aadA1,1 700bp integron carried gene cassette dfr17-aadA5. There were no IntI2 and intI3. Conclusion Integrons are widely carried by Salmonella. Integrons are important molecular mechanism in mediation and dissemination of antibiotic resistance in Salmonella. |
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| Keywords: | Salmonella Integrons Gene cassettes Resistance to antibiotics |
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