温肾阳与降相火对雌性大鼠性激素水平的影响

目的观察有温肾助阳作用的右归丸和有滋阴降相火作用的知柏地黄丸对雌性大鼠血清雌二醇(E2)、孕酮(P)、睾酮(T)水平的影响。方法将大鼠随机分为空白对照组,右归丸大、小剂量组,知柏地黄丸大、小剂量组,用化学发光法测定E2水平,用放免法测定P、T水平。结果知柏地黄丸大剂量组与空白对照组和右归丸大、小剂量组比较,E2水平明显下降(P<0.05);知柏地黄丸小剂量组与右归丸大、小剂量组比较,E2水平明显下降(P<0.05)。知柏地黄丸大剂量组与空白对照组和知柏地黄丸小剂量组比较,P水平明显下降(P<0.05)。各组T水平无差异。结论右归丸对血清E2、P、T水平无影响。大剂量知柏地黄丸可降低血清E2、P水平,对血清T水平无影响。

Influence of therapy of warming kidney yang and lowering ministerial fire on sex hormone level in female rats

Objective To observe the influence of Yougui Pills with the action of warming kidney yang, and Zhibaidihuang Pills with the actions of nourishing yin and lowering ministerial fire, on the serum levels of estradiol (E2), progesterone (P) and testosterone (T) in female rats. Methods All rats were randomly divided into the blank control group, high-dose Yougui Pills group, low-dose Yougui Pills group, high-dose Zhibaidihuang Pills group and low-dose Zhibaidihuang Pills group. The chemiluminescence method was used to detect the level of E2, and the radioimmunity assay was used to determine the levels of P and T. Results The level of E2 in the high-dose Zhibaidihuang Pills group was obviously lower than those in the blank control group, high-dose Yougui Pills group and low-dose Yougui Pills group (P<0.05). The level of E2 in the low-dose Zhibaidihuang Pills group was obviously lower than that in the high-dose Yougui Pills group and low-dose Yougui Pills group (P<0.05). The level of P in the high-dose Zhibaidihuang Pills group was obviously lower than that in the blank control group and low-dose Zhibaidihuang Pills group (P<0.05). The levels of T in all groups were not changed. Conclusion Yougui Pills has no influence on the serum levels of E2, P and T. Zhibaidihuang Pills in high dose can decrease the serum levels of E2 and P, but not influence the level of T.