| 地塞米松及甲氨蝶呤上调HaCaT细胞结合珠蛋白的表达 |
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| 引用本文： | 夏立新,郭哲,崔绍山,王雅坤,陈洪铎. 地塞米松及甲氨蝶呤上调HaCaT细胞结合珠蛋白的表达[J]. 中国麻风皮肤病杂志, 2007, 23(12): 1054-1056 |
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| 作者姓名： | 夏立新 郭哲 崔绍山 王雅坤 陈洪铎 |
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| 作者单位： | 中国医科大学第一医院皮肤科,沈阳,110001 |
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| 摘 要： | 目的:探讨地塞米松及甲氨蝶呤对人角质形成细胞系HaCaT细胞合成结合珠蛋白(Hp)的影响。方法:采用RT-PCR方法检测Hp mRNA表达水平,ELISA方法检测Hp浓度。结果:地塞米松或甲氨蝶呤刺激HaCaT24h后,HaCaT细胞表达Hp增加;但较短刺激时间或较低刺激浓度时,HaCaT细胞表达Hp水平无明显变化。结论:地塞米松及甲氨蝶呤能够上调HaCaT细胞Hp的合成,这种作用具有时间依赖性及剂量依赖性的趋势。
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| 关 键 词： | 结合珠蛋白 地塞米松 甲氨蝶呤 HaCaT细胞 |
| 修稿时间： | 2007-03-07 |
Up-regulation of HaCaT Cell haptoglobin expression in human keratinoc yte cell line by dexamethasone and methotrexate |
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| Xia Lixin,Guo Zhe,Cui Shaoshan,et al.. Up-regulation of HaCaT Cell haptoglobin expression in human keratinoc yte cell line by dexamethasone and methotrexate[J]. China Journal of Leprosy and Skin Diseases, 2007, 23(12): 1054-1056 |
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| Authors: | Xia Lixin Guo Zhe Cui Shaoshan et al. |
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| Affiliation: | Xia Lixin,Guo Zhe,Cui Shaoshan,et al. Department of Dermatology,The First Hospital of China Medical University,Shenyang,110001 |
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| Abstract: | Objective:To determine the impact of dexamethasone and methotrexate on the synthesis haptoglobin (Hp) in human keratinocyte cell line. Methods: The level of Hp mRNA and the concentration of Hp were measured by RT-PCR and ELISA methods respectively. Results: After the stimulation of HaCaT cell by high dose of dexamethasone or methotrexate for 24 hours, the expression of Hp produced by HaCaT cell was increased. If the stimulation time was shorter or the concentration was lower, no significant change of Hp expression was observed. Conclusion: Both dexamethasone and methotrexate can up-regulate the synthesis of Hp of HaCaT cell and this effect may be dose-and time-dependent. |
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| Keywords: | haptoglobin dexamethasone methotrexate HaCaT cell |
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