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| 复方肝毒清对二甲基亚硝胺诱导小鼠肝损伤模型的保护作用 | |
| 引用本文： | 江远,张玲,何金洋,聂广.复方肝毒清对二甲基亚硝胺诱导小鼠肝损伤模型的保护作用[J].中国中西医结合消化杂志,2011,19(1):22-25,I0002. |
| 作者姓名： | 江远 张玲 何金洋 聂广 |
| 作者单位： | 1. 广州医学院附属深圳沙井医院,中医肝病科,广东,深圳,518104 2. 广州中医药大学,热带医学研究所,广东,广州,510405 3. 深圳市第三人民医院,中西医结合科,广东,深圳,518020 |
| 摘 要： | 目的]探讨复方肝毒清对二甲基亚硝胺（Dimethylnitrosamine,DMN）诱导小鼠肝损伤模型的保护作用。方法]将60只小鼠随机分为空白对照组、模型组、联苯双酯组及复方肝毒清高、中、低剂量组。空白对照组予0.85%氯化钠10 ml/kg灌胃,其他各组以15 mg/kg DMN腹腔注射建立小鼠肝损伤模型,同时后4组分别按150mg/kg联苯双酯滴丸和20、10、5 g/kg复方肝毒清灌胃,每12 h给药1次,共4次。赖氏法检测血清丙氨酸氨基转移酶（ALT）、天冬氨酸转氨酶（AST）、清蛋白水平;ELISA法检测血清肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）水平;定量PCR法检测肝组织TNF-α、IL-1βmRNA的表达。结果]复方肝毒清不仅能够显著改善肝功能和肝组织病理,而且能明显降低血清TNF-α、IL-1β水平和肝组织TNF-α、IL-1βmRNA的表达。结论]复方肝毒清可能是通过减少炎症细胞因子的分泌而达到保护肝脏的作用。 |
| 关 键 词： | 肝损伤 肿瘤坏死因子α 白细胞介素1β 中医药疗法 复方肝毒清 |
Protection of compoud Ganduqing Decoction on mouse model of liver injury induced by dimethylnitrosamine |
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| JIANG Yuan,ZHANG Ling,HE Jin-yang,NIE Guang.Protection of compoud Ganduqing Decoction on mouse model of liver injury induced by dimethylnitrosamine[J].Chinese Journal of Integrated Traditional and Western Medicine on Digestion,2011,19(1):22-25,I0002. | |
| Authors: | JIANG Yuan ZHANG Ling HE Jin-yang NIE Guang |
| Institution: | JIANG Yuan1,ZHANG Ling1,HE Jin-yang2,NIE Guang3(1Hepatology Department of Chinese Medicine,Affiliated Shenzhen Shajing Hospital ofGuangzhou Medical College,Shenzhen 518104,China,2Tropical Medicine Instituteof Guangzhou University of Chinese Medicine,Guangzhou 510405,3Department of Integrated Traditional and Western Medicine,theThird Hospital of Shenzhen City,Shenzhen 518020,China) |
| Abstract: | Objective]To investigate the protection of compoud Ganduqing Decoction on mouse model of liver injury induced by dimethylnitrosamine.Methods]Sixty mice were randomly divided into blank control group,model group,dimethyl diphenyl bicarboxylate(DDB)group,compoud Ganduqing high-dose,medium-dose and low-dose groups.Mice of the blank control group were perfused gastrically with normal saline at 10 ml/kg.Other groups for mouse models of liver injury were established by DMN intraperitoneal injection at a dose of... |
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