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| 老年大鼠耳蜗螺旋神经节及耳蜗核线粒体DNA缺失的研究 | |
| 引用本文： | 刘俊,孔维佳,刘维荣. 老年大鼠耳蜗螺旋神经节及耳蜗核线粒体DNA缺失的研究[J]. 听力学及言语疾病杂志, 2005, 13(1): 50-52,i001 |
| 作者姓名： | 刘俊 孔维佳 刘维荣 |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉,430022;湖北省黄石市中心人民医院耳鼻咽喉科,黄石,435000 2. 华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉,430022 3. 湖北省黄石市中心人民医院耳鼻咽喉科,黄石,435000 |
| 基金项目： | 国家自然科学基金资助项目 (编号 3 9670 781) |
| 摘 要： | 目的 探讨耳蜗螺旋神经节及耳蜗核线粒体DNA(mitochondrialDNA ,mtDNA)缺失在老年聋发病中的可能作用。方法 分别测试老年组及青年组大鼠听性脑干反应 (ABR)阈值 ,应用聚合酶链反应 (polymerasechainre action,PCR)技术对老年及青年大鼠耳蜗螺旋神经节及脑组织中耳蜗核mtDNA 4 834bp缺失片段进行检测。结果老年组及青年组大鼠ABR阈值分别为 71.0 0± 6 .75、39.83± 4 .4 5dBpeSPL。老年大鼠耳蜗螺旋神经节及脑组织中耳蜗核mtDNA483 4 缺失出现率分别为 6 6 .7%、10 0 % ;青年鼠耳蜗螺旋神经节及脑组织中耳蜗核均无此片段缺失。 结论 老年大鼠耳蜗螺旋神经节及脑组织中耳蜗核存在mtDNA片段缺失 ,并可能在老年聋发病中起重要作用。 |
| 关 键 词： | 线粒体脱氧核糖核酸 序列缺失 老年聋 大鼠 |
| 文章编号： | 1006-7299(2005)01-0050-03 |
| 修稿时间： | 2004-03-08 |
Mitochondrial DNA Large Deletions in Spiral Ganglion of Cochlear and Cochlear Nucleus of Brain in Elder Rat Associated with Presbyacusis |
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| Liu Jun,Kong Weijia,Liu Weirong. Mitochondrial DNA Large Deletions in Spiral Ganglion of Cochlear and Cochlear Nucleus of Brain in Elder Rat Associated with Presbyacusis[J]. Journal of Audiology and Speech Pathology, 2005, 13(1): 50-52,i001 | |
| Authors: | Liu Jun Kong Weijia Liu Weirong |
| Abstract: | Objective To investigate the possible association of mtDNA deletion in spiral ganglion of cochlear and cochlear nucleus of brain in young and elder rat with presbyacusis.Methods The mtDNA 4834 deletion in spiral ganglion of cochlear and cochlear nucleus of brain in rat was analyed by PCR.Results The deletion rate of mtDNA 4834 in rat with presbyacusis was 66.7% in spiral ganglion, 100% in cochlear nucleus of brain.While no deletion was detected in young rat.Conclusion Deletion in mtDNA of the spiral ganglion of cochlear and cochlear nucleus of brain was found in rat with presbyacusis, and it may play an important role in the pathogens of presbyacusis. |
| Keywords: | Mitochondrial DNA(mtDNA) Sequence deletion Presbyacusis Rat |
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