| 背根神经节来源的雪旺细胞的离体培养 |
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| 引用本文： | 陈益人,周松林,丁斐,顾晓松. 背根神经节来源的雪旺细胞的离体培养[J]. 神经损伤与功能重建, 2007, 2(4): 207-209,221 |
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| 作者姓名： | 陈益人 周松林 丁斐 顾晓松 |
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| 作者单位： | 南通大学江苏省神经再生重点实验室,江苏,南通,226001;南通大学江苏省神经再生重点实验室,江苏,南通,226001;南通大学江苏省神经再生重点实验室,江苏,南通,226001;南通大学江苏省神经再生重点实验室,江苏,南通,226001 |
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| 基金项目： | 南通大学科技处经费基金 |
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| 摘 要： | 目的:优化小鼠雪旺细胞(SCs)的离体培养方法。方法:取新生小鼠的背根神经节,采用酶消化分散法,加入不同的培养液,第1组(NB组)用NB(neuronbasal medium),第2组(FBS组)用含10%FBS的DMEM,继用O4、S-100免疫荧光法染色鉴定SCs阳性细胞,并镜下计数,计算SCs纯度。结果:NB组SCs纯度于第1周为90%,第2周为86.6%;FBS组相应SCs纯度都低于30%。结论:无血清的NB培养液更有利于SCs的增殖和生长。
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| 关 键 词： | 雪旺细胞 背根神经节 离体培养 |
| 文章编号： | 1001-117X（2007）04-0207-03 |
| 修稿时间： | 2007-04-24 |
Cultures of Schwann Cells from Dorsal Root Ganglia of Mice in Vitro |
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| CHE NYi-Ten,ZHOU Song-lin,DING Fei,GU Xiao-song. Cultures of Schwann Cells from Dorsal Root Ganglia of Mice in Vitro[J]. Neural Injury and Functional Reconstruction, 2007, 2(4): 207-209,221 |
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| Authors: | CHE NYi-Ten ZHOU Song-lin DING Fei GU Xiao-song |
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| Affiliation: | Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong University, Nantong 226001, China |
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| Abstract: | Objective:The method of culturing Schwann cells(SCs) from the dorsal root ganglia of mice has been optimized.Methods: SCs were dissociated from the dorsal root ganglia(DRGs) in 1-day-old mice,fed with one of two kind media: Neurobasal media without serum,or DMEM plus 10% FBS.The purity of the SCs was evaluated by percentage of O4 and S-100-immunostaining cells.Results: The purity of SCs in cultures fed with NB reached up to 86%-90%,while the yield of purity fed with 10% FBS was below 30%.Conclusion: Neurobasal media without serum,used for neuronal cultures,is also favorable for SCs growth and/or proliferation in vitro. |
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| Keywords: | Schwann cell dorsal root ganglion culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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