人尿中奥美拉唑代谢物的提取与精制

目的对奥美拉唑在人体内代谢后尿中2种代谢物进行提取与精制后推测其结构并测定其含量。方法24名健康受试者单剂量口服奥美拉唑肠溶胶囊40mg后,收集服药后12h内尿液,用乙醚提取和浓缩,以高效液相色谱法进行分离,将相对纯品进行质谱扫描测定,推测其结构并计算含量。结果经分离得到的2种代谢物推测为吡啶5’—或3’—甲基氧化生成的羟基砜型代谢物、吡啶环上5’—甲基羟化硫醚型代谢物,含量分别为96.54%、97.26%。结论本方法分离得到的奥美拉唑尿中代谢物质纯度较高。

Extraction and Purification of Omeprazole Metabolites in Human Urine

OBJECTIVE:To presume the structures and determine the contents of two metabolites of omeprazole in human urine after extraction and purification.METHODS:24 healthy volunteers were assigned to receive single oral dose of 40mg omepazole capsules,whose urinary samples collected within 12 hours after administration were extracted and concentrated with diethyl ether,and separated by HPLC.The relative purified metabolites were detected by mass spectrum,the structures of which were presumed and the contents were computed.RESULTS: The isolated 2 metabolites of omeprazole were presumed to be pyridine 5'— or 3'— methyl oxidation-generated hydroxy sulfone metabolite and 5'—methy hydroxylation thioether metabolite in pyridine ring,and the contents of which were 96.54% and 97.26%,respectively.CONCLUSION:The metabolites of omeprazole isolated from urinary samples by the method mentioned above were of high purity.