| Hsa-mir-216a真核表达载体的构建及其在胃癌细胞SGC7901/DDP中的表达 |
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| 引用本文： | 徐红美,许文林,姚俊,冷加燕,吴晓君,陈巧云. Hsa-mir-216a真核表达载体的构建及其在胃癌细胞SGC7901/DDP中的表达[J]. 江苏大学学报(医学版), 2011, 21(4): 289-293 |
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| 作者姓名： | 徐红美 许文林 姚俊 冷加燕 吴晓君 陈巧云 |
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| 作者单位： | 江苏大学附属人民医院中心实验室,江苏,镇江,212002 |
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| 基金项目： | 国家自然科学基金资助项目，江苏省卫生重大课题基金资助项目 |
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| 摘 要： | 目的:构建人mir-216a真核表达载体,实现其在胃癌细胞SGC7901/DDP中的表达,为进一步研究mir-216a的功能打下基础.方法:依据miRBase数据库中pre-mir-216a序列设计引物;PCR扩增pre-mir-216a基因并将其克隆至pGenesil-1载体;将pGenesil-1-hsa-mir-...
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| 关 键 词： | mir-216a 真核表达载体 SGC7901/DDP细胞 转染 |
Construction of hsa-mir-216a eukaryotic expression vector and its expression in gastric cancer cells SGC7901/DDP |
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| XU Hong-mei,XU Wen-lin,YAO Jun,LENG Jia-yan,WU Xiao-jun,CHEN Qiao-yun. Construction of hsa-mir-216a eukaryotic expression vector and its expression in gastric cancer cells SGC7901/DDP[J]. Journal of Jiangsu University Medicine Edition, 2011, 21(4): 289-293 |
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| Authors: | XU Hong-mei XU Wen-lin YAO Jun LENG Jia-yan WU Xiao-jun CHEN Qiao-yun |
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| Affiliation: | (Central Laboratory,the Affiliated People′s Hospital of Jiangsu University,Zhenjiang Jiangsu 212002,China) |
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| Abstract: | Objective: To construct a recombinant eukaryotic expression vector of hsa-mir-216a and to express it in gastric cancer SGC7901/DDP cells for detecting its effects.Methods: Primers were designed based on the pre-mir-216a sequence in miRBase database;the pre-mir-216a gene was amplified by PCR and cloned into pGenesil-1 vector.The gastric cancer SGC7901/DDP cells were transfected with pGenesil-1-hsa-mir-216a vector and the expression of mature miR-216a was examined in SGC7901/DDP cells by semi-quantitative RT-... |
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| Keywords: | mir-216a |
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