Osteoclast formation from mouse bone marrow cells on micro/nano-scale patterned surfaces |
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| Affiliation: | 1. Department of Biomaterials and Bioengineering, Faculty of Dental Medicine, Hokkaido University, Sapporo, 060-8586, Japan;2. Research and Development Division, Hokkaido University Hospital Clinical Research and Medical Innovation Center, Sapporo, 060-8648, Japan;3. Immune Signal Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, 904-0495, Japan;4. Department of Molecular Cell Pharmacology, Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Kita-ku, Sapporo, 060-8586, Japan;5. School of Life Science and Technology, Tokyo Institute of Technology, 4259 B51, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan;6. Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, Sapporo, 060-8586, Japan;1. Department of Oral Biochemistry, Asahi University School of Dentistry, Mizuho, Gifu 501-0296, Japan;2. Department of Operative Dentistry, Asahi University School of Dentistry, Mizuho, Gifu 501-0296, Gifu 500-8471, Japan;3. Chemistry Laboratory, Department of Business Administration, Asahi University School of Business Administration, Mizuho, Gifu 501-0296, Japan;4. Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Mizuho, Gifu 501-0296, Japan;5. AKITAYA HONTEN CO., LTD., R&D, Gifu 500-8471, Japan;6. Chemistry Laboratory, Asahi University School of Dentistry, Mizuho, Gifu 501-0296, Gifu 500-8471, Japan;7. Department of Scientific and Linguistic Fundamentals for Nursing, Osaka City University Graduate School of Nursing, Osaka 558-8585, Japan;1. Department of Oral Functional Anatomy, Graduate School of Dental Medicine, Hokkaido University, Kita13 Nishi7, Kita-ku, Sapporo 060-8586, Japan;2. Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Kita13 Nishi7, Kita-ku, Sapporo 060-8586, Japan;1. Department of Oral Anatomy, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan;2. Department of Oral Maxillofacial Radiology, School of Dentistry, Aichi Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya, Aichi, 464-8651, Japan;3. Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya, Aichi, 464-8651, Japan;4. Department of Dental Hygiene, Aichi Gakuin University Junior College, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan;1. Division of Clinical Chemistry, Niigata University Graduate School of Health Sciences, Niigata 951-8518, Japan;2. Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan;3. Division of Anaerobic Research, Life Science Research Center, Gifu University, Gifu 501-1194, Japan |
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| Abstract: | ObjectivesOsteoclasts can sense the surface topography of materials. However, it is difficult to identify the structural factors that affect osteoclast formation and its function. Furthermore, we hypothesized that the type of osteoclast precursor cells also affects osteoclastogenesis in the materials. In this study, we investigated the effects of defined micro/nanoscale patterns on osteoclastogenesis from bone marrow cells (BMCs).MethodsVarious cyclo-olefin polymer (COP) patterns were prepared using nanoimprinting. The effects of shape, size, and height of the patterns, and the wettability of the patterned surfaces on osteoclastogenesis from BMCs were evaluated in vitro.ResultsOsteoclast formation was promoted on pillars (diameter, 1 μm or 500 nm; height, 500 nm). Notably, osteoclastogenesis from BMCs was better promoted on hydrophobic pillars than on hydrophilic pillars. In contrast, decreased osteoclast formation was observed on the nanopillars (diameter, 100 nm; height, 200 nm).ConclusionsWe demonstrated the promotion of osteoclast formation from BMCs on hydrophobic pillars with diameters of 1 μm and 500 nm. Some cellular behaviors in the patterns were dependent on the type of osteoclast precursor cells. The designed patterns are useful for designing the surface of dental implants or bone replacement materials with a controllable balance between osteoblast and osteoclast activities. |
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| Keywords: | Bone marrow cells Micro/nanoscale patterns Osteoclast formation Pillar BMC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0035" }," $$" :[{" #name" :" text" ," _" :" bone marrow cell PBMCs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" peripheral blood mononuclear cells COP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" cyclo-olefin polymer TCPS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" tissue culture polystyrene α-MEM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" α-minimum essential medium FBS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" fetal bovine serum M-CSF" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" macrophage colony-stimulating factor TRAP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" tartrate-resistant acid phosphatase |
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