Abstract: | SUMMARY
Stenotrophomonas maltophilia contains a novel
chromosomally-encoded qnr gene named Smqnr that
contributes to low intrinsic resistance to quinolone. We described
Smqnr in 13 clinical isolates of S.
maltophilia from two Brazilian hospitals, over a 2-year period. The
strains were identified by API 20 NE (bioMérieux, France). Susceptibility by
microdilution method to trimetroprim/sulfamethoxazole, ciprofloxacin,
levofloxacin, minocycline, ceftazidime, chloramphenicol and
ticarcillin/clavulanate was performed according to CLSI. PCR detection of
Smqnr gene was carried out. The sequence of
Smqnr was compared with those deposited in GenBank.
Pulsed-field gel electrophoresis (PFGE) of all strains was performed. Thirteen
Smqnr positives isolates were sequenced and three novel
variants of Smqnr were identified. All 13 Smqnr
isolates had distinguishable patterns by PFGE. This is the first report of
Smqnr in S. maltophilia isolated in
Brazil. |