The expression patterns of MALAT-1, NEAT-1, THRIL,and miR-155-5p in the acute to the post-acute phase of COVID-19 disease |
| |
| Institution: | 1. Iran University of Medical Sciences, Deputy of Health, Tehran, Iran;2. Tabriz University of Medical Sciences, Infectious and Tropical Diseases Research Center, Tabriz, Iran;3. Iran University of Medical Sciences, School of Medicine, Department of Virology, Tehran, Iran;4. University of Medical Sciences, Institute of Immunology and Infectious Diseases, Research Center of Pediatric Infectious Diseases, Tehran, Iran;5. Tabriz University of Medical Sciences, Center for Women''s Health Research Zahra, School of Medicine, Department of Pathology, Tabriz, Iran;6. Tehran University of Medical Sciences, School of Medicine, Department of Clinical Biochemistry, Tehran, Iran;7. Iran University of Medical Sciences, School of Nursing and Midwifery, Department of Medical Surgical Nursing, Tehran, Iran |
| |
| Abstract: | IntroductionOne of the hallmarks of COVID-19 is overwhelming inflammation, which plays a very important role in the pathogenesis of COVID-19. Thus, identification of inflammatory factors that interact with the SARS-CoV-2 can be very important to control and diagnose the severity of COVID-19. The aim of this study was to investigate the expression patterns of inflammation-related non-coding RNAs (ncRNAs) including MALAT-1, NEAT-1, THRIL, and miR-155-5p from the acute phase to the recovery phase of COVID-19.MethodsTotal RNA was extracted from Peripheral Blood Mononuclear Cell (PBMC) samples of 20 patients with acute COVID-19 infection and 20 healthy individuals and the expression levels of MALAT-1, NEAT-1, THRIL, and miR-155-5p were evaluated by real-time PCR assay. Besides, in order to monitor the expression pattern of selected ncRNAs from the acute phase to the recovery phase of COVID-19 disease, the levels of ncRNAs were re-measured 6?7 weeks after the acute phase.ResultThe mean expression levels of MALAT-1, THRIL, and miR-155-5p were significantly increased in the acute phase of COVID-19 compared with a healthy control group. In addition, the expression levels of MALAT-1 and THRIL in the post-acute phase of COVID-19 were significantly lower than in the acute phase of COVID-19. According to the ROC curve analysis, these ncRNAs could be considered useful biomarkers for COVID-19 diagnosis and for discriminating between acute and post-acute phase of COVID-19.DiscussionInflammation-related ncRNAs (MALAT-1, THRIL, and miR-150-5p) can act as hopeful biomarkers for the monitoring and diagnosis of COVID-19 disease. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
