山西省恙虫病调查与病原学研究

目的：１９９５年山西晋南地区发生恙虫病流行，我们对该地区人群进行了流行病学调查；并对从患者和野鼠体内分离的恙虫病立克次体进行了病原学研究。方法：利用微量间接免疫荧光法（ＭＩＦ）对恙虫病立克次体在人群中的流行进行了调查；采用ＭＩＦ、ＰＣＲ／ＲＦＬＰ和序列分析方法对恙虫病立克次体分离株进行了鉴定。结果和结论：晋南地区人民群血清恙虫病立克次体抗体阳性率为２５％；恙虫病立克次体山西分离株（Ｓｘｈ９５１，Ｓｘｈ９５２，Ｓｘｈ９５３，Ｓｘｍ９７）的血清型别为Ｇｉｌｌｉａｍ株；序列分析提示：在相对分郭质量５６０００蛋白基因水平上，Ｓｘｈ９５１株与Ｙｏｎｇｃｈｏｎ株亲缘关系最近。

Investigation of scrub typhus in Shanxi Province and study on its etiology

Objective: To investigate the epidemiology of scrub typhus in people of Shanxi province during an epidemic in this area in 1995 ,and to characterize the 4 Rickettsia tsutsugamushi strains isolated(Sxh951,Sxh952,Sxh953,Sxm97).Methods: Microimmuno fluoresence method was used to investigate the epidemic of R.tsutsugamushi and microimmuno fluoresence, PCR/RFLP and sequence analysis were performed to characterize these isolates. Results and conclusion: The positive rate of antibodies against R.tsutsugamushi in people of this area was 25%. The serotype of these isolates was Gilliam strain and sequence analysis suggested that in respect of M r 56 000 protein gene Sxh951 strain was closest to Yongchon strain in homology, which was isolated from South Korea. [