| 低剂量甲氨蝶呤对DNA的损伤及甲酰四氢叶酸对其保护作用 |
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| 引用本文： | 杨建一,李莉,彭芸,杜圣家. 低剂量甲氨蝶呤对DNA的损伤及甲酰四氢叶酸对其保护作用[J]. 癌变．畸变．突变, 2004, 16(1): 24-26. DOI: 10.3969/j.issn.1004-616x.2004.01.007 |
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| 作者姓名： | 杨建一 李莉 彭芸 杜圣家 |
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| 作者单位： | 山西医科大学基础医学院，山西 太原 030001 |
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| 摘 要： | 背景与目的：研究多次低剂量应用甲氨蝶呤所致BALB/c小鼠淋巴细胞及骨髓细胞DNA的损伤，并探讨甲酰四氢叶酸对其损伤的保护作用。材料与方法：应用甲氨蝶呤0.5 mg/kg腹腔注射，每周1次，共8周；用单细胞凝胶电泳方法检测其外周血淋巴细胞及骨髓细胞DNA的损伤情况。结果：单用甲氨蝶呤组细胞尾长及拖尾频率与对照组相比均有显著差异，而同时应用等量的甲酰四氢叶酸，其细胞尾长及拖尾频率与对照组相比无显著差异。结论：SCGE技术可敏感地检测多次应用低剂量甲氨蝶呤造成的DNA损伤，并能反映甲酰四氢叶酸对甲氨蝶呤所致的DNA损伤的保护作用。
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| 关 键 词： | 甲氨蝶呤 甲酰四氢叶酸 单细胞凝胶电泳 DNA损伤 |
| 文章编号： | 1004-616X(2004)01-0024-03 |
| 收稿时间： | 2003-08-20 |
| 修稿时间： | 2003-08-20 |
DNA Damage Caused by low Dose Methotrexate and Protective Effect of Folinic acid |
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| YANG Jian-yi,LI Li,PENG Yun,et al. DNA Damage Caused by low Dose Methotrexate and Protective Effect of Folinic acid[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2004, 16(1): 24-26. DOI: 10.3969/j.issn.1004-616x.2004.01.007 |
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| Authors: | YANG Jian-yi LI Li PENG Yun et al |
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| Affiliation: | Shanxi Medical University, Taiyuan 030001，China |
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| Abstract: | BACKGROUND & AIM: To study the DNA damage of BALE/ c mice caused by multiple treatment with low-dose methotrexate (MTX)and the protective effect of folinic acid (FA). MATERIAL AND METHODS: After ip MTX at dose of 0.5 mg/kg once a week for 8 weeks, DNA damage of peripheral lymphocytes and bone marrow cells were examined by single cell gel electrophoresis(SCGE) . RESULTS: There were signifi cant differences of the tail length and the frequency of tailing cells between MTX and the control. However, there were no signifi cant differences between MTX + FA and the control. CONCLUSION: SCGE is a rapid, simple and sensitive technique for detecting DNA damage caused by low dose methotrexate and folinic acid could protect peripheral lymphocytes and bone marrow cells DNA from damage induced by MTX. |
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| Keywords: | methotrexate folinic acid single cell gel electrophoresis(SCGE) DNA damage |
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