脑损伤诱导神经细胞凋亡的动物实验研究

为探讨脑损伤诱导神经细胞凋亡的作用及其机制,应用大鼠液压冲击脑损伤模型,在脑损伤后不同时间段（３ｈ,１２ｈ,２４ｈ,４８ｈ,７２ｈ）处死动物,分别应用ＨＥ染色、流式细胞仪检测、ＴＵＮＥＬ法测定、ＤＮＡ凝胶电泳分析,免疫组化研究神经细胞凋亡的改变。结果显示：动物经中度脑损伤处理后,其受伤各时间段脑组织可见凋亡的神经细胞及ＴＵＮＥＬ阳性细胞,ＤＮＡ呈现“梯状”断裂现象,细胞凋亡百分率为９．８％￣１４．

An experimental study on traumatic brain injury for inducing neuronal apoptosis in rats]

This study was designed to explore the effect of traumatic brain injury and its mechanism for inducing neuronal apoptosis. The model of experimental traumatic brain injury was used. The rats were dispatched at different times(3 h, 12 h, 24 h, 48 h, 72 h) after traumatic brain injury, and the neuronal apoptosis was evaluated with microscope (HE staining), TUNEL method, flow cytometry, gel electrophoresis and immunohistochemistry assay. The results showed that the brain tissue neurons treated by traumatic brain injury underwent morphological changes of apoptosis, the DNA presented "ladder" break, the rate of neuronal apoptosis at different times ranged from 9.8% to 14.0%, but that of the control group was 1.7% and the expressions of related apoptosis genes(c-myc, fas and fasL) were increased. The findings of this study indicate that traumatic brain injury can induce neuronal apoptosis, and its molecular mechanism might be related to the expression of some apoptosis genes.