艾灸对胃黏膜损伤大鼠胃黏膜表皮生长因子、转化生长因子-α及其受体的影响

目的:观察艾灸对应激性胃黏膜损伤大鼠胃黏膜表皮生长因子(EGF)、转化生长因子-α(TGF-α)与表皮生长因子受体(EGFR)的影响,探讨艾灸修复胃黏膜损伤的作用机制。方法:SD大鼠随机分为正常组、模型组、艾灸组,每组12只。束缚冷应激法制作应激性胃黏膜损伤大鼠模型,在造模之前,艾灸组选取"足三里""中脘""脾俞""胃俞"行艾灸预处理8d。酶联免疫法检测胃黏膜组织中EGF、TGF-α的含量,免疫组化法检测胃黏膜EGFR蛋白表达。结果:与正常组比较,模型组大鼠胃黏膜TGF-α的含量明显增加(P<0.05);与模型组比较,艾灸组大鼠胃黏膜EGF、TGF-α的含量及EGFR蛋白表达明显上调(P<0.05,P<0.01)。结论:预先艾灸"足三里""中脘""脾俞""胃俞"可上调应激性胃黏膜损伤大鼠胃黏膜EGF、TGF-α的含量及EGFR表达,可能与其对胃黏膜的保护作用有关。

Effects of moxibustion on gastric mucosal EGF and TGF-alpha contents and epidermal growth factor receptor expression in rats with gastric mucosal lesion

Objective To observe the effects of moxibustion on contents of epidermal growth factor(EGF) and transforming growth factor-alpha(TGF-α) and epidermal growth factor receptor(EGFR) expression in the gastric mucosa tissue in rats with gastric mucosal lesion.Methods Thirty-six SD rats were randomly and equally divided into control,model and moxibustion groups.Gastric mucosal lesion model was duplicated by restraint and cool water immersion stress.Pre-moxibustion was applied to "Zusanli"(ST36) and "Zhongwan"(CV12),"Pishu"(BL20) and "Weishu"(BL21) alternately,once everyday for 8 days before modeling.The contents of EGF and TGF-α in gastric mucosa were detected by enzyme-linked immunosorbent assay(ELISA) and the expression of EGFR determined by immunohistochemistry.Results Compared with the control group,only TGF-α content in the gastric mucosa in the model group was increased significantly(P<0.05).Compared with the model group,the EGF and TGF-α contents and EGFR immunoactivity in the gastric mucosa were increased significantly in the moxibustion group(P<0.05,P<0.01).Conclusion Pre-moxibustion at ST36,CV12,BL20 and BL21 can up-regulate gastric mucosal EGF and TGF-α contents and EGFR protein expression in gastric mucosa lesion rats,which may contribute to its effect in relieving stress-induced gastric mucosal injury.