| Correlation of Seven Biological Factors （Hsp90a,p53, MDM2, Bcl-2, Bax,Cytochrome C,and Cleaved caspase3） with Clinical Outcomes of ALK＋ Anaplastic Large-cell Lymphoma |
| |
| 引用本文： | Li HL,Huang XP,Zhou XH,Ji TH,Wu ZQ,Wang ZQ,Jiang HY,Liu FR,Zhao T. Correlation of Seven Biological Factors （Hsp90a,p53, MDM2, Bcl-2, Bax,Cytochrome C,and Cleaved caspase3） with Clinical Outcomes of ALK＋ Anaplastic Large-cell Lymphoma[J]. Biomedical and environmental sciences : BES, 2011, 24(6): 630-641. DOI: 10.3967/0895-3988.2011.06.007 |
| |
| 作者姓名： | Li HL Huang XP Zhou XH Ji TH Wu ZQ Wang ZQ Jiang HY Liu FR Zhao T |
| |
| 作者单位： | [1]Department of Pathology, Nanfang Hospital affiliated to Southern Medical University, Guangzhou 510515 Guangdong, China [2]Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzho 510515, Guangdong, China |
| |
| 基金项目： | This research was supported by the National Natural Science Foundation of China （No. 30901169）. We thank Prof. GU Long Jun （Department of Hematology, Xinghua Hospital affiliated to Shanghai Jiao Tong University, Shanghai） and Prof. HOU Jian （Department of Hematology, Changzheng Hospital affiliated to the Second Military Medical University, Shanghai） for kindly providing Karpas299 and SH-1 cell lines; Prof. HUANG Wei Yi （Faculty of Life Sciences of Nanjing Agricultural College, Nanjing） for herbimycin A drug. |
| |
| 摘 要： |
|
| 关 键 词： | Bax蛋白 细胞色素C BCL-2 P53蛋白 临床疗效 MDM2 ALK 淋巴瘤 |
| 收稿时间： | 2011-04-08 |
Correlation of seven biological factors (Hsp90a, p53, MDM2, Bcl-2, Bax, Cytochrome C, and Cleaved caspase3) with clinical outcomes of ALK+ anaplastic large-cell lymphoma |
| |
| Li Hui Ling,Huang Xue Ping,Zhou Xin Hua,Ji Tian Hai,Wu Zi Qing,Wang Zhi Qiang,Jiang Hui Yong,Liu Fan Rong,Zhao Tong. Correlation of seven biological factors (Hsp90a, p53, MDM2, Bcl-2, Bax, Cytochrome C, and Cleaved caspase3) with clinical outcomes of ALK+ anaplastic large-cell lymphoma[J]. Biomedical and environmental sciences : BES, 2011, 24(6): 630-641. DOI: 10.3967/0895-3988.2011.06.007 |
| |
| Authors: | Li Hui Ling Huang Xue Ping Zhou Xin Hua Ji Tian Hai Wu Zi Qing Wang Zhi Qiang Jiang Hui Yong Liu Fan Rong Zhao Tong |
| |
| Affiliation: | Department of Pathology, Nanfang Hospital affiliated to Southern Medical University, Guangzhou 510515 Guangdong, China. |
| |
| Abstract: | ObjectiveTo explore correlation of seven apoptosis-related proteins (Hsp90a, p53, MDM2, Bcl-2, Bax, Cytochrome C, and Cleaved caspase3) with clinical outcomes of ALK+ anaplastic large-cell lymphoma (ALCL).MethodsUsing immunohistochemistry and immunofluorescence double staining methods, the expressions of these seven apoptosis-associated proteins were studied to clarify their relationship with clinical outcomes of 36 ALK+ and 25 ALK-systemic ALCL patients enrolled between 1996 and 2006. The relationship of these apoptosis-regulating proteins with NPM-ALK status was also evaluated with the tyrosine inhibitor herbimycin A (HA) in vitro by immunocytochemistry, Western blotting and flow cytometric assays.ResultsThe presence of Hsp90α-, MDM2-, Bax-, Cytochrome C, and Cleaved caspase3-positive tumor cells was found significantly different in ALK+ and ALK-ALCLs, which was correlated with highly favorable clinical outcome. The Bcl-2- and p53-positive tumor cells were found in groups of patients with unfavorable prognosis. Inhibition of NPM-ALK by HA could reactivate the p53 protein and subsequent apoptosis-related proteins and therefore induced apoptosis in ALK+ ALCL cells.ConclusionOur results suggest that these seven proteins might be involved in apoptosis regulation and associated with clinical outcome of ALK+ systemic ALCLs. We also reveal a dynamic chain relation that NPM-ALK regulates p53 expression and subsequent apoptosis cascade in ALK+ ALCLs. |
| |
| Keywords: | ALK＋ALCL NPM-ALK Prognostic factors Apoptosis Herbimycin A |
| 本文献已被 维普 ScienceDirect PubMed 等数据库收录！ |
|
