茶多酚对人鼻咽癌细胞裸鼠移植瘤的生长抑制作用

目的：通过观察茶多酚在体内对人鼻咽癌细胞的抑制作用，探讨其可能的抗癌机制。方法以人鼻咽癌细胞株HONE1建立裸鼠皮下移植瘤模型，以茶多酚腹腔注射干预，在观察抑瘤率基础上，以实时荧光定量PCR及Western blot法分别检测裸鼠移植瘤VEGF在mRNA及蛋白水平上的表达变化。结果茶多酚具有显著抑制鼻咽癌细胞 HONE1移植瘤生长的作用，与模型对照组相比，低、高剂量茶多酚组体内抑瘤率分别为18．82％、47．66％，呈浓度相关性（P＜0．05）；荧光定量 PCR及Western blot结果示，茶多酚可下调VEGF的mRNA和蛋白表达水平，且呈浓度依赖性，其中高剂量组变化明显，差异有统计学意义（P＜0．05）。结论茶多酚对人鼻咽癌HONE1细胞裸鼠移植瘤的生长具有明显抑制作用，机制可能与调节VEGF表达，从而抑制肿瘤血管生成等作用有关。

Inhibitive effect of tea polyphenol on the growth of human nasopharyngeal carcinoma cell xenograft in nude mice

Objective To evaluate the inhibitive effect of tea polyphenol on the growth of human nasopharyngeal carcinoma HONE1 cell xenograft in nude mice ,and to explore the underlying mechanisms .Methods Tumor model was established by subcu‐taneous inoculation of nasopharyngeal carcinoma cell HONE1 into nude mice ,was used to evaluate the antitumor effect of tea poly‐phenol in vivo .The expression levels of VEGF were detected by real‐time PCR and western blot .Results The growth of xenograft in nude mice was significantly suppressed after application of tea polyphenol at a dose‐dependent manner .To compare with control group ,the inhibition rates were 18 .82% (P<0 .05) and 47 .66% (P<0 .05)when treated at low and high dose respectively ,With in‐creased concentration of TP ,the inhibition rates increased .Real‐time fluorescence quantitative‐PCR and western blot results showed that the expression of VEGF decreased at a dose‐dependent manner .The change of high dose group was obviously ,the difference was statistically significant(P<0 .05) .Conclusion Tea polyphenol could significantly inhibit the growth of human nasopharyngeal carcinoma HONE1 cell xenograft in nude mice ,probably by down regulating the VEGF protein level to inhibit tumor angiogenesis effects .