外周血CD4＋T 细胞组蛋白乙酰化在狼疮性肾炎中的作用

目的：研究狼疮性肾炎（LN）患者外周血CD4＋ T细胞组蛋白乙酰化水平，探究其在L N发病中的作用。方法18例L N患者均来自该科病房，全部患者都达到美国风湿学会1997年推荐的系统性红斑狼疮（SLE）的分类诊断标准。满足以下条件为活动性的LN，蛋白尿大于0．5g／d，或活动性尿沉渣改变（血尿红细胞超过5个／Hp，或脓尿白细胞大于5个／Hp，或细胞管型大于1个／Hp），血肌酐升高大于1．2mg／L，排除感染、肾结石和其他原因引起的。非活动的LN ：蛋白尿小于0．5g／L，非活动性的尿沉渣。将L N患者分为两组：非活动性（I组）8例、活动性的L N组（A组）10例，对照组（N组）8例。分别采集L N患者和N组的外周血50 mL ，密度梯度离心法（Ficoll法）分离出外周血中的单个核细胞，采用免疫磁珠技术分选出CD4＋ T细胞，并提取组蛋白，采用组蛋白H3／H4乙酰化检测试剂盒检测组蛋白H3／H4的乙酰化水平，分析组蛋白H3／H4乙酰化水平及与疾病的关系。结果（1）与N组相比，A和I组LN患者外周血CD4＋ T细胞的组蛋白 H3、H4均呈低乙酰化状态（P＜0．01）；（2）A组的组蛋白H4的乙酰化水平低于I组（P＜0．01），而组蛋白 H3乙酰化水平两组间差异无统计学意义（P＞0．05）。（3）组蛋白 H4的乙酰化水平与24 h尿蛋白排泄量呈负相关（r＝－0．661，P＜0．05）。结论在LN患者中的CD4＋ T细胞组蛋白 H3和组蛋白 H4均呈低乙酰化状态可能参与了LN的发病，CD4＋ T细胞组蛋白H4的乙酰化水平可能与LN的活动性有关。

The histone acetylation of CD4+T cells of peripheral blood in the lupus nephritis

Objective To study the histone acetylation level of CD4+ T cells in peripheral blood of lupus nephritis ,to explore the role of histone acetylation in pathogenesis of lupus nephritis .Methods According to Feng X ,Bernstein ,Wagner S J and other scholars′s classification criteria for LN ,those who met the following conditions considered for the activity of LN :proteinuria >0 .5 g/d ,change or activity of urinary sediment (hematuria > 5 red blood cell /Hp ,or pyuria > 5 Hp white blood cells ,or 1 cell type/Hp) ,serum creatinine increased >1 .2 mg/L ,and the exclusion of infection ,kidney stones and other causes .Lupus nephritis pa‐tients were divided into inactive group (group I) 8 people ,active group (group A) 10 people .18 patients with LN and 8 normal con‐trols were collected peripheral blood 50 mL ,density gradient centrifugation method (Ficoll method) for separation of mononuclear cells in peripheral blood;CD4+ T cell was analyzed by immunomagnetic beads ,extracted histone acetylation level and detected H3/H4 protein by the acetylation of histone H3/H4 kits and the relationship of histone H3/H4 acetylation with diseases was analyzed . Results First ,compared with group N ,the histone H3 and H4 of CD4+ T cells in peripheral blood both in A and I of group LN pa‐tients showed low acetylation status (P<0 .01);Second ,the acetylation level of histone H4 in group A was lower than that in group I (P<0 .01) ,histione H3 acetyl the level of the group of two groups had no statistical significant (P>0 .05) .Third ,it was nega‐tively related to the acetylation level of histone H4 and 24 h urinary protein excretion(r= -0 .661 ,P<0 .05) .Conclusion Histone H3 and histone H4 of the CD4+ T cells showed low acetylation may be involved in the pathogenesis of lupus nephritis .The acetyla‐tion level of histone H4 in CD4+ T cells may be related to the activity of the LN .