芦荟大黄素对高转移乳腺癌细胞MDA-MB-231体外转移潜能的影响

目的研究芦荟大黄素(Aloe emodin,AE)对人高转移乳腺癌细胞MDA-MB-231体外转移潜能的影响及其作用机制。方法 MTT法检测AE对MDA-MB-231细胞增殖的抑制作用;Transwell chamber法检测AE对MDA-MB-231细胞侵袭重组基底膜能力和趋化性运动能力的影响;RT-PCR、Western blot法检测AE对MDA-MB-231细胞黏着斑激酶(FAK)mRNA和蛋白表达的影响。明胶酶谱法检测MDA-MB-231细胞分泌的基质金属蛋白酶-9(MMP-9)活性。结果 80μmol·L-1 AE抑制MDA-MB-231细胞体外侵袭重组基底膜能力、趋化性运动能力,其抑制率分别为(52.98±5.46)%,(45.88±8.51)%。作用于MDA-MB-231细胞24 h后,AE下调FAK mRNA和蛋白表达,下调MDA-MB-231细胞分泌MMP-9。结论 AE抑制MDA-MB-231细胞体外侵袭能力、趋化性运动能力,其作用机制与其下调FAK表达和MMP-9的分泌有关。

Effect of Aloe emodin on metastatic abilities of human high metastatic breast cancer MDA-MB-231 cells in vitro

Aim To investigate the effect of Aloe emodin(AE) on metastasis-associated abilities of human high metastatic breast cancer MDA-MB-231 cells in vitro.Methods MTT assay was used to evaluate the cell viability of MDA-MB-231 cells after 6h AE treatment.The effect of AE on the invasion and migration of MDA-MB-231 cells was determined by transwell chamber assay.RT-PCR,Western blot were applied to detect the expression levels of FAK mRNA and protein in MDA-MB-231 cells.The secretion of matrix metalloproteinase-9(MMP-9) from MDA-MB-231 cells was detected by zymography assay after AE treatment.Results AE significantly inhibited the invasion and migration of MDA-MB-231 cells in vitro,the inhibitory rates were(52.98±5.46)%,(45.88±8.51)% after 6h treatment with 80 μmol·L-1 AE.After 24h treatment,AE down-regulated the expression levels of FAK mRNA and protein of MDA-MB-231 cells.The secretion of MMP-9 from MDA-MB-231 cells was inhibited by 24h AE treatment.Conclusion AE can inhibit the invasion and migration of MDA-MB-231 cells.Its possible mechanism may be involved in the deduction of the expression level of FAK and the production of MMP-9.