体现中医药特色的道地药材黄连品质评价系统研究

本文以道地药材黄连的研究为例,对其生长环境进行分析,运用ISSR等方法考察其遗传型,采用HPLC、1H-NMR及NIR等方法对化学型进行分析,并从抗内毒素、抗微生物的生物热力学,以及治疗糖尿病相关的胰岛素抵抗生物效应等角度考察其药效型,综合分析其相关性,初步建立了基于黄连生态型、基因型、化学型和药效型的道地药材品质评价方法,同时建立了结合道地性的黄连规范化质量标准,为构建体现中医药特色的黄连品质评价方法提供了科学依据。

Research on Quality Evaluation Method of Geo-authentic Medicinal Herb Rhizoma Coptidis for the Reflection of Outstanding Feature of TCM

This study took geo-authentic medicinal material Rhizoma Coptidis as an example and analyzed soil characteristics of the growing environment of Rhizoma Coptidis . The methods of ISSR, HPLC, 1H-NMR and NIR were respectively used for exploring its genotype and investigating on its chemitype. In the respects of anti-endotoxin, anti-bacterial activities and insulin resistance that was related to the treatment of diabetes, the pharmacodynamic type was studied and the relationship was comprehensively analyzed. The quality evaluation of geo-authentic medicinal materials was preliminarily established based on ecotype, genotype chemitype and pharmacodynamic type of Rhizoma Coptidis . Simultaneously, quality standards of Rhizoma Coptidis were set up in combination with genuine character, which provided the scientific basis for establishing Chinese medicine characteristic methods of quality evaluation of Rhizoma Coptidis .