| 不同品种石斛的HPLC-DAD-ELSD指纹图谱研究 |
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| 引用本文： | 石媛慧,郭力,许莉,郝普彦,陈佳江,韦练,罗方利,张廷模,韩瑜.不同品种石斛的HPLC-DAD-ELSD指纹图谱研究[J].世界科学技术-中医药现代化,2013,15(6):1329-1332. |
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| 作者姓名： | 石媛慧 郭力 许莉 郝普彦 陈佳江 韦练 罗方利 张廷模 韩瑜 |
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| 作者单位： | 成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;成都中医药大学药学院/教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地 成都 611137;四川万安石斛产业开发有限公司 成都 610213 |
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| 基金项目： | 科学技术部国家“十二五”科技支撑计划项目（2011BAI13B02-8）：石斛规范化种植基地及其SOP 优化、升级研究，负责人：张廷模。 |
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| 摘 要： | 目的:采用HPLC-DAD-ELSD建立石斛指纹图谱,并对金钗、鼓槌、叠鞘、细茎和反瓣等5种石斛的指纹图谱进行对比研究。方法:采用Dikma C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.5%冰乙酸水溶液,梯度洗脱,柱温30℃,流速1 mL·min-1,DAD串联蒸发光散射检测器检测。结果:建立5种石斛的HPLC指纹图谱,并标定9个指纹峰,5种石斛相似度较低。结论:建立的指纹图谱方法稳定、可靠、重现性好,可直接反映石斛品种与化学成分变化的关系。
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| 关 键 词： | 石斛 指纹图谱 HPLC-DAD-ELSD |
| 收稿时间： | 2012/10/22 0:00:00 |
| 修稿时间： | 2012/11/27 0:00:00 |
Study on HPLC-DAD-ELSD Fingerprint in Different Species of Dendrobii Caulis |
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| Shi Yuanhui,Guo Li,Xu Li,Hao Puyan,Chen Jiajiang,Wei Lian,Luo Fangli,Zhang Tingmo and Han Yu.Study on HPLC-DAD-ELSD Fingerprint in Different Species of Dendrobii Caulis[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2013,15(6):1329-1332. |
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| Authors: | Shi Yuanhui Guo Li Xu Li Hao Puyan Chen Jiajiang Wei Lian Luo Fangli Zhang Tingmo and Han Yu |
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| Institution: | Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, The Breeding Base of State Key Laboratory of Resources Systems Research And Development Utilization of Chinese Herbal Medicines Co-constructed by The Ministry of Science and Technology of The P.R.C. and Sichuan Province, Chengdu 611137, China;Sichuan Wan'an Dendrobium Industry Development Co. Ltd., Chengdu 610213, China |
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| Abstract: | |
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| Keywords: | Cervical spondylosis vertigo Mongolian medicine bloodletting therapy cupping therapy occipital vein |
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