| 反义寡核苷酸抑制端粒酶活性对喉癌细胞Hep-2增殖的影响 |
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| 引用本文： | 蒲一平,刘勇,吴家廉,曹前.反义寡核苷酸抑制端粒酶活性对喉癌细胞Hep-2增殖的影响[J].重庆医科大学学报,2002,27(2):160-162. |
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| 作者姓名： | 蒲一平 刘勇 吴家廉 曹前 |
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| 作者单位： | 1. 重庆医科大学第二临床学院耳鼻咽喉科,重庆,400010
2. 贵州安顺市第二人民医院五官科,安顺,561000 |
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| 摘 要： | 目的:检测端粒酶的反义寡核苷酸对喉癌细胞的影响。Hep-2方法:采用法及平板集落形成实验检测靶向于端MTT粒酶的反义寡核苷酸对喉癌细胞株增殖的抑制作用。用法检测反义寡核苷酸对喉癌细胞株细胞内端粒Hep-2 PCR-ELISAHep-2酶活性的影响。结果:反义寡核苷酸明显抑制了喉癌细胞的增殖,此作用有剂量、时间依赖性。反义寡核苷酸可抑制喉Hep-2癌细胞端粒酶活性,此作用有浓度依赖性。结论:靶向于端粒酶的反义寡核苷酸能抑制喉癌细胞的增殖。Hep-2
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| 关 键 词： | 喉癌 反义寡核苷酸 端粒酶 |
| 文章编号： | 0253-3626(2002)02-0160-03 |
| 修稿时间： | 2001年9月25日 |
Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of laryngeal carcinoma cells |
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| PU Yiping,et al.Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of laryngeal carcinoma cells[J].Journal of Chongqing Medical University,2002,27(2):160-162. |
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| Authors: | PU Yiping |
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| Abstract: | Objective: To investigate the effect of the antisense oligodeoxynucleotide (AS-ODN) targeting against telomerase on laryngeal carcinoma cells Hep-2 strain in vitro. Methods: MTT test and plat clone forming test were used to measure the proliferation inhibition of the AS-ODN targeting against telomerase on Hep-2 cell strain. Telomerase activity of Hep-2 cell strain was analysed by PCR-ELISA technique. Results: The AS-ODN inhibited Hep-2 cell strain obviously with dose dependence and time dependence. The AS-ODN inhibited telomerase activity of Hep-2 cell strain with density dependence. Conclusion: The AS-ODN targeting against telomerase could inhibit the proliferation of Hep-2 cell strain. |
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| Keywords: | Laryngeal carcinoma Antisense oligodeoxynucleotide Telomerase |
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