红景天苷减轻叠氮钠诱导线粒体损伤的作用

目的观察红景天苷对呼吸链复合体IV抑制剂叠氮钠(NaN₃)诱导的线粒体损伤的保护作用，探讨其在防治神经退行性疾病中可能的作用机制。方法将叠氮钠与人神经母细胞瘤细胞株SH-SY5Y共同孵育，MTT法测定细胞存活力，JC-1法检测线粒体膜电位变化。刃天青法检测大鼠脑线粒体功能。结果64 mmol·L^-1叠氮钠与SH-SY5Y共同孵育4 h后，细胞存活率明显下降，线粒体膜电位下降。预先加入红景天苷能明显提高细胞存活率，维持线粒体膜电位。650 μmol·L^-1叠氮钠能使大鼠脑线粒体功能下降，预先加入红景天苷能明显改善线粒体功能。结论红景天苷能够减轻叠氮钠(NaN₃)诱导的线粒体损伤，能够改善线粒体功能，这可能是其抗老年痴呆的机制之一。

Effect of salidroside on mitochondria injury induced by sodium azide

AimTo study the protective effect of salidroside on mitochondria injury induced by sodium azide. MethodsHuman neuroblastoma SH-SY5Y cells were exposed to sodium azide with different concentration of salidroside, then cell viability was measured by thiazolyl blue (MTT) method and mitochondrial membrane potential (MMP) was detected by JC-1 method. Protective effect of salidroside against disfunction of mitochondria induced by sodium azide was detected by resazurin method. ResultsAfter exposing to 64 mmol·L^-1 sodium azide for 4 hours, cell viability and MMP of SH-SY5Y cells significantly decreased. When pretreated with salidroside, the cell damage was greatly reduced and the mitochondrial membrane potential was maintained. Furthermore, salidroside can protect function of rat brain mitochondria against damage induced by sodium azide. ConclusionSalidroside was demonstrated to play an important role in improving the function of mitochondria.