应用分子杂交技术对“子宫颈癌高危患者”筛查及随访的临床—病理学研究

目的：应用分子杂交技术对子宫颈癌高危患者进行筛查及随访的临床—病理学研究。方法：应用缺口翻译法３２Ｐ—标记的ｈＰＶ１６，１８，ｃ－Ｈａ－ｒａｓ－１及ｃ－ｍｙｃ－ＤＮＡ与子宫颈组织中的ＤＮＡ进行分子杂交，并通过Ｘ线胶片显示杂交结果。结果：慢性子宫颈炎组织中ｈＰＶ１６，１８，ｃ－Ｈａ－ｒａｓ－１及ｃ－ｍｙｃ阳性２９例，经１年随访，４例病情加重，２例手术治疗，２例保守治疗。４种高危因素均为阴性者随访２０例，均未发现病情加重。结论：用分子杂交技术筛查及随访子宫颈炎患者，对早期发现子宫颈癌具有重要意义。

Clinic pathological study on screening and following up of the "high risk patients with cervical cancer" by using molecular hybridization technique

Objective To study the clinicalpathological features on screening and following up of the "high risk patients with cervical cancer" by using molecular hybridization technique. Methods Molecular hybridization was used with 32 p labelled hPV 16,18,c Ha ras 1,c myc DNA and DNA in cervical tissue showed by X ary film autography. Results 29 cases were of hPV 16,hPV 18,c Ha ras and c myc positive in cervical tissues.They were followed up for 1 year,4 cases of chronic cervitis were more severe than one year ago.2 cases were operated, another 2 cases were being treated.Cervical cancers were not developed in 20 cases of negative for high risk factors. Conclusion Screening and following up for the high risk patients with cervical cancer by using molecular hybridization technique is currently significant in predictative diagnosis of cervical cancer.