白花丹素对骨肉瘤MG-63细胞迁移的影响及其作用机制

目的 观察白花丹素对骨肉瘤细胞迁移的抑制作用并初步探讨其可能的机制.方法 以2.5 μmol/L、5μmol/L和10 μmol/L浓度白花丹素分别作用于骨肉瘤MG-63细胞,以含0.1％ DMSO完全培养基为对照组.作用24 h后,Transwell法观察骨肉瘤MG-63细胞迁移能力,Real-time PCR检测骨肉瘤MG-63细胞中Ezrin基因mRNA表达情况,Westem blot检测Ezrin蛋白和p-Ezrin的表达情况.结果 Transwell结果显示,作用24h后,各实验组下层小室细胞数明显减少,2.5 μmol/L组、5μmol/L组和10 μmol/L组的细胞迁移率分别为(69.9±4.5)％、(39.3±3.5)％和(26.2±4.1)％,呈浓度依赖性(P＜0.05);Real-time PCR结果显示,骨肉瘤MG-63细胞中Ezrin mRNA表达水平明显下降,呈浓度依赖性(P＜0.05).Western blot结果显示,Ezrin蛋白和p-Ezrin的表达量明显降低,呈浓度依赖性(P＜0.05).结论 白花丹素能够抑制骨肉瘤MG-63细胞迁移,其作用机制可能与抑制骨肉瘤MG-63细胞中Ezrin的表达及活化有关.

Effects of plumbagin on invasion in osteosarcoma cell line MG-63 and its possible mechanisms

Objective To assess the ability of plumbagin to inhibit invasion by osteosarcoma and explore possible mechanisms. Methods Osteosarcoma MG-63 cultures were treated for 24 h with 0.1% DMSO only（control） or with plumbagin at 2.5，5 and 10 μmol/L. Then the invasive ability of the MG-63 cells was measured in a trans-well assay，changes in Ezrin mRNA levels were determined using real-time PCR，and changes in Ezrin and p-Ezrin protein levels were assessed using Western blot assay. Results Plumbagin treatment reduced the number of invading artificial basement cells in a dose-dependent manner，and rate of cell migration at the three plumbagin doses was respectively，69.9±4.5%，39.3±3.5% and 26.2±4.1% of control cells（P<0.05）. Real-time PCR showed that plumbagin significantly reduced level of mRNA in a dose-dependent manner（P<0.05）. Similarly，Western blotting showed that plumbagin significantly reduced levels of Ezrin and p-Ezrin in a dose-dependent manner. Conclusion Plumbagin may inhibit tissue invasion by osteosarcoma MG-63 cells，and it may do so in part by suppressing Ezin and p-Ezrin expression.