iNOS在弥漫大B细胞淋巴瘤组织中的表达及临床意义

目的 探讨诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）在弥漫大B细胞淋巴瘤（diffuse large B cell lymphoma，DLBCL）组织中的表达及临床意义。方法 用免疫组化法检测40例DLBCL及15例反应性增生淋巴结中iNOS的表达情况，分析iNOS的表达与DLBCL患者性别、年龄、临床分期、国际预后指数（IPI）之间的关系。结果 iNOS在DLBCL及反应性增生淋巴结组织中的阳性表达率分别为67.5%、26.7%，两者比较差异有统计学意义（P<0.05）。iNOS的表达水平与临床分期、IPI有关（P<0.05），与患者性别、年龄无关（P>0.05）。结论 iNOS在DLBCL组织中有较高的阳性表达，其表达水平与患者的临床分期及IPI相关，提示iNOS在DLBCL的发生、发展过程中起重要作用。

Expression of iNOS in diffuse large B cell lymphoma and its clinical significance

Objective To assess the expression of inducible nitric oxide synthase（iNOS）　in diffuse large B cell lymphoma，and to clarify the relationship between iNOS expression and clinicopathological factors. Methods Paraffin-embedded tissue arrays were prepared from 40 patients with diffuse large B cell lymphoma and 15 patients with reactive lymph node proliferation. Immuno-histochemistry was used to detect iNOS expression，and possible relationships of expression with gender，age，disease stage and International prognostic index （IPI） were analyzed. Results The rate of tissues positive for iNOS was 67.5% in diffuse large B cell lymphoma but only 26.7% in reactive lymph node proliferation. Expression levels of iNOS were significantly higher in diffuse large B cell lymphoma tissues than in reactive lymph node proliferation（P<0.05），and these levels correlated with histology grade and IPI （both P<0.05），but not with gender，age，or disease stage（P>0.05）. Conclusion Diffuse large B cell lymphoma is associated with a higher rate of iNOS expression， and this correlates with histology grade and IPI. Therefore iNOS appears to play an important role in the development of this type of lymphoma.