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血清β—羟丁酸测定方法的建立及其在糖尿病酮症酸中毒中的意义
摘    要:

关 键 词:β-羟丁酸 糖尿病 酮症酸中毒 血清诊断

Establishment of method for determining serum beta-hydroxybutyric acid and its meaning in diabetes ketosis acidism]
B Yi,G F Wei,Y Tang. Establishment of method for determining serum beta-hydroxybutyric acid and its meaning in diabetes ketosis acidism][J]. Bulletin of Hunan Medical University, 2001, 26(2): 185-186
Authors:B Yi  G F Wei  Y Tang
Affiliation:Department of Clinical Laboratory, Xiangya Hospital, Centeal South University, Changsha 410008, China.
Abstract:OBJECTIVE: This study was to establish enzyme-rate method for determining serum beta-hydroxybutyric acid, and to discuss its meaning in diabetes ketosis acidism. METHODS: Enzyme-rate method was used to determine serum beta-hydroxybutyric acid in 60 cases of normals, 30 cases of diabetes, and 8 cases of ketosis acidism by Hitachi 7170A autochemistry analyzer. RESULTS: The recovery rate is 108%, the linerity extent 0-4.0 mmol.L-1, the coefficient of variation within-run is 2.2% and day-to-day is 2.6%. CONCLUSION: The enzyme-rate method for determining serum beta-hydroxybutyric acid save time, gives accurate results and has wide linerity, so it can used for diagnosis of diabetes ketosis acidism.
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