BacT／ALERT 3 D检测技术应用于结核分枝杆菌快速培养的效果评价

目的：评价BacT/ALERT 3D快速培养系统对结核分枝杆菌（MTB）的检测和肺结核早期诊断的应用价值。方法采用BacT/ALERT 3D液体培养技术和L-J固体培养法分别对病例标本培养，比较2种方法检出结果及检出时间。结果平均出现阳性天数BacT/ALERT 3D法为（13±2.05）d，L-J固体培养法为（34.7±4.76）d，差异有统计学意义（P＜0.05）；BacT/ALERT 3D法和L-J固体培养法对治疗2月后肺结核病例的涂阳标本MTB培养阳性检出率分别为71.18％（42/59）和67.80％（40/59），而对涂阴标本BacT/ALERT 3D法 MTB培养阳性率39.62％（42/106）高于L-J固体培养法阳性率26.42％（28/106），差异有统计学意义（P＜0.05）。结论与传统L-J固体培养法比较，BacT/ALERT 3D液体培养技术明显缩短检出时间，并能提高低浓度（痰涂阴）标本的结核分枝杆菌阳性检出率。

The effect evaluation on the application of BacT/ALERT 3 D technique for the fast cultivation of mycobacterium tuberculosis

Objective To evaluate the BacT/ALERT 3D liquid rapid culture system for the rapid detection of mycobacterium tuberculosis（MTB）and early diagnosis of pulmonary tuberculosis.Methods BacT/ALERT 3D liquid culture mediums and lowenstein-jensen（L-J）solid culture mediums were applied to detect Mycobacterium tuberculosis sputum specimens respectively.Analysis of detection results and detection time were also performed.Results Average positive days of BacT/ALERT 3D liquid culture medium was（13 ±2.05）days,which was shorter than that of L-J solid culture mediums（34.7 ±4.76 ）d （P〈0.05 ）.Compared to the L -J solid culture mediums,BacT/ALERT 3D liquid culture medium had higher positive rate for 59 patients whose sputum smear test was positive,and the positive rate were 71.18%（42/59）and 67.80%（40/59）respectively（P〈0.05）.BacT/ALERT 3D liquid culture medium had higher positive rate than L-J solid culture mediums for 106 patients whose sputum smear test was negative,and the positive rate were 39.62%（42/106）and 26.42%（28/106）respectively（P〈0.05）.Conclusion Compared to the traditional L-J solid culture system,BacT/ALERT 3D liquid culture system can shorten detection time and improve the positive detection rate of MTB in specimens with low concentration.