浙江省鼠形动物蜱源立克次体感染状况调查

目的了解浙江省不同类型地理环境中鼠形动物蜱源立克次体的感染状况。方法 2009—2011年在安吉县、金东区和天台县用夹夜法捕获鼠形动物,进行分类鉴定、无菌采集肝脾标本,并采用巢式PCR方法检测无形体属与埃立克体属16SrRNA以及立克次体属（包括斑疹伤寒群和斑点热群）与东方体属热休克蛋白基因groEL。结果 3个调查点共捕获鼠形动物14种851头,社鼠（30.32%）、黑线姬鼠（18.80%）和青毛鼠（11.75%）为主要优势种,其中金东区、安吉县和天台县的优势鼠种分别为社鼠（39.20%）、青毛鼠（32.05%）和黑线姬鼠（59.57%）。562份肝脾标本中检出立克次体48份,阳性检出率为8.54%;其中无形体属占3.38%,斑疹伤寒群占1.78%,恙虫病东方体属占1.78%,埃立克体属占1.07%,斑点热群占0.53%。金东区和安吉县无形体属检出率较高为4.76%和4.27%,斑点热群仅在天台县检出。社鼠的立克次体阳性检出率最高为14.97%。同一鼠形动物可存在多种立克次体混合感染。结论浙江省不同类型地理环境鼠形动物中广泛存在立克次体感染,不同地理位罝和不同鼠形动物的蜱源立克次体检出率不同。

A Cross-sectional study on tick-borne Rickettsiae infections among murine-like animals

Objective To investigate the status of tick- borne Rickettsiae infections among murine- like animals in different areas of Zhejiang province. Methods Liver and spleen samples of murine- like animals captured through night trapping method were collected from Anji,Jinhua and Tiantai County according to their geographic locations and historical detection of Rickettsiae. Nest- PCR tests were used to determine the presence of the 16S rRNA genes of Anaplasma and Ehrlichia,and the heat shock protein genes（ groEL） of Rickettsiae（ including typhus and spotted fever group） and Orientia in these samples. Results A total of 851 murine- like animals belonging to 14 species were captured. The predominant species were Rattus confucianus（ 30. 32%）,Apodemus agrarius（ 18. 80%） and Thallomys paedulcus（ 11.75%） and they were significantly different among three areas（ P〈0.05）. 48 Rickettsia positive were found in 562tested samples with the positive rate of 8. 54%,among which the percentage of Anaplasma,typhus group Rickettsia,Orientia,Ehrlichia and spotted fever group Rickettsia were 3. 38%,1. 78%,1. 78%,1. 07% and 0. 53% respectively.The positive rates of Anaplasma in Jindong（ 4. 76%） and Anji（ 4. 27%） were significantly higher than that in Tiantai（ P〈0. 05） while the spotted fever group Rickettsia were found only in Tiantai County. Moreover,Rattus confucianus- the predominant species of Zhejiang Province- had the highest infection rate of tick- borne Rickettsiae up to 14. 97%. Co-infections with several Rickettsiae were existed among the same species. Conclusion Rickettsiae infections exist widely among different areas of Zhejiang province and the positive rates are significantly different among species.