石墨烯量子点荧光猝灭-恢复法测定Cu2+与谷胱甘肽的含量

通过在温和条件下氧化石墨粉的方法合成高分散性、高荧光强度的石墨烯量子点(GQDs)，并运用此GQDs对Cu²⁺和GSH具有荧光猝灭-恢复的效应，建立对这两种物质简便、快捷的检测方法。Cu²⁺与GSH的浓度分别在1.0~10.0 mmol/L、0.1~1.0 mmol/L范围内与GQDs的荧光强度呈良好线性关系，检测限分别为0.01和0.1 mmol/L。此外，在实际样品的检测中，加标法测得的回收率分别为93%~101%、96%~107%。该方法操作简便，测定准确，精密度高，且常见的金属离子及潜在共存物质对Cu²⁺与GSH的检测均没有干扰。

Detection of copper ions and glutathione based on off-on fluorescent graphene quantum dots

We developed a rapid method to detect Cu²⁺ and glutathione(GSH)based on the fluorescence quenching-recovery properties of graphene quantum dots(GQDs), which were synthesized by the gentle oxidation of graphite powder. The results revealed that the fluorescence intensity of GQDs versus concentration of Cu²⁺ and GSH had good linearity, with the detection limits of 0. 01 and 0. 1 mmol/L, respectively. The fluorescence quenching was linearly proportional to the concentrations of Cu²⁺ ranging from 1. 0 to 10. 0 mmol/L; the fluorescence intensity was linearly enhanced with the concentrations of GSH ranging from 0. 1 to 1. 0 mmol/L. In addition, the method was successfully applied to the determination of real samples with recoveries falling between 93%-101% and 96%-107%, respectively. This method is simple, accurate and precise. There was no interference with other familiar co-existing metal ions and potential materials.