| 多种细胞因子在CD34+细胞增殖分化中的作用 |
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| 引用本文： | 王强,王冰,李荷莲,王巍.多种细胞因子在CD34+细胞增殖分化中的作用[J].吉林大学学报(医学版),2004,30(2):250-252. |
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| 作者姓名： | 王强 王冰 李荷莲 王巍 |
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| 作者单位： | 吉林大学第二医院妇产科,吉林,长春,130041;吉林大学中日联谊医院整形科,吉林,长春,130031;吉林大学第一医院康复科,吉林,长春,130021 |
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| 摘 要： | 目的:探讨血小板生成素（TPO）等多种细胞因子在分离后的脐血造血干/祖细胞体外扩增中的作用。方法:在集落培养体系及体外液体扩增体系中，分别用含有和不含有TPO细胞因子组合实验，比较其结果。结果:含有TPO组其诱导红系爆式集落单位(BFU-E
)和粒巨细胞集落形成单位(GFU-GM)集落形成倍数、细胞总数和CD34+细胞增殖倍数明显高于不含有TPO组。
结论：TPO与干细胞因子(SCF)、白细胞介素3(IL-3)等其他细胞因子协同可明显提高早期造血干/祖细胞扩增效率。
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| 关 键 词： | 胎血 细胞因子类 CD34+细胞 细胞分化 |
| 文章编号： | 1671-587X(2004)02-0250-02 |
| 收稿时间： | 2002-05-13 |
| 修稿时间： | 2002年5月13日 |
Effects of many kinds of cytokines on CD34+ cell proliferation and differentiation |
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| WANG Qiang ,WANG Bing ,LI He lian ,WANG Wei.Effects of many kinds of cytokines on CD34+ cell proliferation and differentiation[J].Journal of Jilin University: Med Ed,2004,30(2):250-252. |
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| Authors: | WANG Qiang WANG Bing LI He lian WANG Wei |
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| Affiliation: | WANG Qiang 1,WANG Bing 2,LI He lian 1,WANG Wei 3 |
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| Abstract: | ObjectiveTo study thrombopoietin (TPO) in the expansion of cord blood hematopoietic stem/projenitor cells in vitro . MethodsIn the colony culture system and the liquid expansion in vitro , different cytokines combination groups with or without TPO were used to observe the result. ResultsThe proliferation folds of colony BFU E and colony CFU GM, the total cells and CD34 cells in the groups with TPO were in obviously higher than those in the groups without TPO ( P <0 05). ConclusionTPO can improve the expansion effect of the early hematopoietic stem/projenitor cells obviously with SCF,IL 3 and other traditional cytokines. |
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| Keywords: | fetal blood cytokines CD34 cells cell differentiation |
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