| EXPRESSION OF PEDF mRNA AND PROTEIN IN NORMAL MOUSE RETINA AND EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION TISSUES |
| |
| 引用本文： | 张雷 王康孙 王玲 张士胜 石海云. EXPRESSION OF PEDF mRNA AND PROTEIN IN NORMAL MOUSE RETINA AND EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION TISSUES[J]. 上海第二医科大学学报, 2004, 16(1): 27-32 |
| |
| 作者姓名： | 张雷 王康孙 王玲 张士胜 石海云 |
| |
| 作者单位： | DepartmentofOphthalmology,Ruijinhospital,SSMU,Shanghai200025,China |
| |
| 摘 要： | Objective To study the expression of pigment epithelium derived factor (PEDF) in normal mouse retina and experimental choroidal neovascularization (CNV) tissues. Methods CNV mouse models were induced by diode laser. The expression of PEDF mRNA and protein in normal mouse retina and CNV tissues were detected by in situ hybridization and immunohistochemical study. Results In normal mouse retina, PEDF mRNA was observed in the ganglion cell layer, inner nuclear layer and RPE cell layer, and PEDF protein was observed mainly in the nerve fiber layer, ganglion cell layer, photoreceptor cell layer and RPE cell layer, and lower level expression of PEDF protein was also observed in the inner plexiform layer and outer plexiform layer. In CNV tissues, the expression of PEDF mRNA and protein was also observed. 3d and 1 week after photocoagulation, the expression level of PEDF was relatively lower, and increased following the development of CNV. The level was the highest 2 weeks after photocoagulation, then decreased at 3 weeks. Conclusion PEDF was expressed in different layers of retina and was obviously expressed in the CNV tissues induced by laser photocoagulation. These findings suggest that PEDF may participate and modulate the development of CNV.
|
| 关 键 词： | 色素上皮源因子 视网膜 脉络膜 蛋白表达 动物模型 免疫组织化学 |
EXPRESSION OF PEDF mRNA AND PROTEIN IN NORMAL MOUSE RETINA AND EXPERIMENTAL CHOROIDAL NEO-VASCULARIZATION TISSUES |
| |
| ZHANG Lei WANG Kang-sun WANG Ling ZHANG Shi-shengSHI Hai-yun. EXPRESSION OF PEDF mRNA AND PROTEIN IN NORMAL MOUSE RETINA AND EXPERIMENTAL CHOROIDAL NEO-VASCULARIZATION TISSUES[J]. Journal of Shanghai Second Medical University(Foreign Language Edition), 2004, 16(1): 27-32 |
| |
| Authors: | ZHANG Lei WANG Kang-sun WANG Ling ZHANG Shi-shengSHI Hai-yun |
| |
| Affiliation: | ZHANG Lei WANG Kang-sun WANG Ling ZHANG Shi-shengSHI Hai-yunDepartment of Ophthalmology,Ruijin hospital,SSMU,Shanghai 200025,China |
| |
| Abstract: | Objective To study the expression of pigment epithelium derived factor (PEDF) in normalmouse retina and experimental choroidal neovascularization (CNV) tissues. Methods CNV mouse models were induced by diode laser. The expression of PEDF mRNA and protein in normal mouse retina and CNV tissues were detected by in situ hybridization and immunohistochemical study. Results In normal mouse retina,PEDF mRNA was observed in the ganglion cell layer, inner nuclear layer and RPE cell layer, and PEDF protein was observed mainly in the nerve fiber layer, ganglion cell layer, photoreceptor cell layer and RPE cell layer, and lower level expression of PEDF protein was also observed in the inner plexiform layer and outer plexiform layer. In CNV tissues, the expression of PEDF mRNA and protein was also observed. 3d and 1 week after photocoagulation, the expression level of PEDF was relatively lower, and increased following the development of CNV. The level was the highest 2 weeks after photocoagulation, then decreased at 3 weeks. Conclusion PEDF was expressed in different layers of retina and was obviously expressed in the CNV tissues induced by laser photocoagulation. These findings suggest that PEDF may participate and modulate the development of CNV. |
| |
| Keywords: | pigment epithelium derived factor choroidal neovascularization retina |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
