高效液相色谱法同时测定金嗓开音丸中绿原酸和连翘苷的含量

目的建立HPLC法同时测定金嗓开音丸中绿原酸？连翘苷含量。方法采用HPLC法,色谱柱：DikmaDiamonsil C18柱,流动相：乙腈-0.085%磷酸,流速：1.0 mL.min-1,柱温：30℃,检测波长：280 nm。结果绿原酸进样量在0.037 04-0.370 4μg与峰面积线性关系良好,r=0.999 69,平均加样回收率为99.02%,RSD为0.82%（n=9）;连翘苷进样量在0.020 66-0.206 6μg与峰面积线性关系良好,r=0.999 48,平均加样回收率为98.81%,RSD为1.01%（n=9）。结论本法简便、准确、重现性好,能排除其他成分的干扰,可用于该产品质量控制。

Simultaneous determination of content of chlorogenic acid and phillyrin in Jinsangkaiyin pills by HPLC

Objective To establish an HPLC method for the simultaneous determination of content of chlorogenic acid and phillyrin in Jinsangkaiyin pills.Methods C18 column was used with acetonitrile and 0.085% phosphoric acid solution as the mobile phase,the flow rate was 1.0 mL·min-1,the column temperature was 30 ℃ and the detection wavelength was 280 nm.Results The calibration curves were linear within 0.037 04～0.370 4 μg（r=0.999 69） for chlorogenic acid,and 0.020 66～0.206 6 μg（r=0.999 48） for phillyrin.The average recovery of them was 99.02%（RSD=0.82%,n=9） and 98.81%（RSD=1.01%,n=9）,respectively.Conclusion The method is specific,accurate and reproducible,and can be used for quality control.