The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals

Objective

The present study evaluated the use of a reagent to stabilize the DNA extractedfrom human dental tissues stored under different temperature conditions and timeintervals.

Material and Methods

A total of 161 teeth were divided into two distinct groups: intact teeth andisolated dental pulp tissue. The samples were stored with or without the productat different time intervals and temperature. After storage, DNA extraction andgenomic DNA quantification were performed using real-time PCR; the fragments ofthe 32 samples that represented each possible condition were analyzed to find thefour pre-selected markers in STR analysis.

Results

The results of the quantification showed values ranging from 0.01 to 10,246.88ng/μL of DNA. The statistical difference in the quantity of DNA was observed whenthe factors related to the time and temperature of storage were analyzed. Inrelation to the use of the specific reagent, its use was relevant in the group ofintact teeth when they were at room temperature for 30 and 180 days. The analysisof the fragments in the 32 selected samples was possible irrespective of theamount of DNA, confirming that the STR analysis using an automated method yieldsgood results.

Conclusions

The use of a specific reagent showed a significant difference in stabilizing DNAin samples of intact human teeth stored at room temperature for 30 and 180 days,while the results showed no justification for using the product under the otherconditions tested.