bFGF对体外关节软骨细胞创伤愈合与增殖的影响

目的：探讨碱性成纤维细胞生长因子（ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｆａｃｔｏｒ，ｂＦＧＦ）对关节软骨细胞创伤愈合与增殖行为的影响。方法；采用体外创伤愈合模型及ＭＴＴ比色方法，对软骨细胞创伤愈合与增殖行为进行分析。结果：ｂＦＧＦ浓度为５ｎｇ／ｍｌ时，即可显著促进软骨细胞创伤愈合，浓度为５０ｎｇ／ｍｌ时，其促进作用达到最大值。

Effects of Basic Fibroblast Growth Factor on Wound Healing and Proliferation of Chondrocytes in Vitro

Objective: To study the effects of basic fibroblast growth factor(bFGF) on wound healing and proliferation of chondrocytes.Methods:The wound healing and proliferation of chondrocytes were assayed in a healing model in vitro and by MTT colorimetric method respectively.Results:bFGF significantly enhanced the wound healing of chondrocytes at the concentration of 5ng/ml,and this enhancement was maximal in the presence of 50ng/ml bFGF.bFGF also enhanced the proliferation of chondrocytes.1ng/ml bFGF significantly increased the proliferation of chondrocytes in the second passage,but had no effect in the fifth passage until the bFGF concentration got to 10ng/ml.Increasing the bFGF to 100ng/ml did not show additional improvement of proliferation in both passages.Conclusions:bFGF can enhance wound healing and proliferation of chondrocytes in vitro,but the proliferation response of chondrocytes in different passages may be different,the enhancement of wound healing by bFGF may be related to the cell proliferation response.