| Olig1过表达对大鼠神经干细胞向少突胶质细胞分化的影响 |
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| 引用本文： | 吕合作,邹健,王艳霞,陆佩华.Olig1过表达对大鼠神经干细胞向少突胶质细胞分化的影响[J].神经解剖学杂志,2010,26(2). |
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| 作者姓名： | 吕合作 邹健 王艳霞 陆佩华 |
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| 作者单位： | 1. 蚌埠医学院第一附属医院中心实验室,蚌埠,233004;上海交通大学医学院神经生物学实验室,上海,200025
2. 上海交通大学医学院神经生物学实验室,上海,200025 |
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| 摘 要： | 目的:Olig1基因修饰的神经干细胞(NSCs)移植治疗中枢神经脱髓鞘疾病具有重要的应用前景。本研究目的是构建大鼠olig1真核表达载体,并观察其过表达对大鼠NSCs向少突胶质细胞分化的影响。方法:采用RT-PCR,以新生大鼠脊髓RNA为模板,扩增olig1基因,定向克隆到pEGFP-N3载体中;用电穿孔方法转染pEGFP-N3-olig1表达载体至NSCs中,然后用RT-PCR鉴定olig1的表达,免疫荧光染色鉴定NSCs向少突胶质细胞的分化情况。结果:成功构建了pEGFP-N3-olig1真核表达载体,olig1在重组质粒转染的NSCs中能够高效表达。重组质粒转染的NSCs在体外诱导分化后,能够较空质粒转染的NSCs产生更多的少突胶质细胞(P0.01)。结论:Olig1过表达能够显著促进大鼠NSCs向少突胶质细胞方向分化。
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| 关 键 词： | 基因表达 克隆 Olig1 神经干细胞 大鼠 |
Effect of olig1 overexpression on differentiation of rat neural stem cells into oligodendrocytes |
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| Lü Hezuo,Zou Jian,Wang Yanxia,Lu Peihua.Effect of olig1 overexpression on differentiation of rat neural stem cells into oligodendrocytes[J].Chinese Journal of Neuroanatomy,2010,26(2). |
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| Authors: | Lü Hezuo Zou Jian Wang Yanxia Lu Peihua |
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| Abstract: | Objective:Olig1 gene-modified neural stem cells (NSCs) transplantation is promising in the treatment of central nervous system demyelinating disease. Our main purpose was to construct rat olig1 eukaryotic expression plasmid and detect its effect of overexpression on differentiation of rat NSCs into oligodendrocytes. Methods:The RT-PCR was used to amplify rat olig1 gene from RNA of neonatal rat spinal cord. The olig1 gene was inserted into eukaryotic expression vector pEGFP-N3 to construct recombinant expres... |
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| Keywords: | Olig1 gene expression cloning Olig1 neural stem cells rats |
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