| 七叶皂苷体外抗SGC-7901细胞的作用及机制研究 |
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| 引用本文： | 吴子侠,吴海竞,曹莉,孙晓飞,明志君,张学光.七叶皂苷体外抗SGC-7901细胞的作用及机制研究[J].中国药理学通报,2009,25(1). |
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| 作者姓名： | 吴子侠 吴海竞 曹莉 孙晓飞 明志君 张学光 |
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| 作者单位： | 苏州大学医学生物技术研究所,江苏,苏州,215123 |
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| 基金项目： | 国家自然科学基金，苏州市三药科技攻关资助项目，苏州大学博士学位人员资助项目 |
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| 摘 要： | 目的研究七叶皂苷体外抗SGC-7901肿瘤细胞的作用及其机制。方法采用MTT法观察药物对SGC-7901肿瘤细胞的增殖抑制作用;采用流式细胞仪检测SGC-7901肿瘤细胞周期的变化;FITC-AnnexinⅤ/PI双标记检测SGC-7901肿瘤细胞的凋亡;Western blot检测凋亡相关蛋白Bcl-2的表达。结果七叶皂苷可明显抑制SGC-7901肿瘤细胞的增殖,并抑制细胞周期和诱导细胞发生凋亡,可下调凋亡相关蛋白Bcl-2的表达。结论七叶皂苷体外对SGC-7901肿瘤细胞具有良好的抑制作用,并可诱导细胞发生凋亡。
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| 关 键 词： | 七叶皂苷 细胞凋亡 SGC-7901肿瘤细胞 |
Studies of the mechanism and inhibitory effect of aescine on SGC-7901 cells in vitro |
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| WU Zi-xia,WU Hai-jing,CAO Li,SUN Xiao-fei,MING Zhi-jun,ZHANG Xue-guang.Studies of the mechanism and inhibitory effect of aescine on SGC-7901 cells in vitro[J].Chinese Pharmacological Bulletin,2009,25(1). |
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| Authors: | WU Zi-xia WU Hai-jing CAO Li SUN Xiao-fei MING Zhi-jun ZHANG Xue-guang |
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| Abstract: | Aim To study the mechanism and inhibi-tory effect of Aescine on SGC-7901 cells in vitro.Methods MTT method was used to detect SGC-7901 proliferation.Flow cytometer method was used to determine cell cycles.AnnexinⅤ-FITC apoptosis derection kit was used to detect apoptosis of cells.Western blot was employed to analyze the expression of Bcl-2.Results MTT result showed that Aescine could significantly inhibit the growth of SGC-7901.AnnexinⅤ-FITC apoptosis derection kit and Western blot results suggested that Aescine could induce the apoptosis of SGC-7901.Conclusions Aescine can inhibit the growth of SGC-7901 and induce the apoptosis of cells in vitro. |
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| Keywords: | Aescine apoptosis SGC-7901 |
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