| PCR技术检测HBV DNA评价 |
| |
| 引用本文： | 郑真,李新建,杨双旺,胡亚琴,李晓华. PCR技术检测HBV DNA评价[J]. 临床消化病杂志, 1999, 11(3): 116-117 |
| |
| 作者姓名： | 郑真 李新建 杨双旺 胡亚琴 李晓华 |
| |
| 作者单位： | 1. 解放军第四五四医院,南京,210002
2. 南京炮兵学院门诊部 |
| |
| 摘 要： | 目的 为评价聚合酶链反应（ＰＣＲ）技术检测ＨＢＶ ＤＮＡ较之传统酶联免疫吸附测定法（ＥＬＩＳＡ）的优越性，评价ＨＢｓＡｇ阴性消化科住院病人携带ＨＢＶ的状态，方法 我们使用ＰＣＲ技术检测了１１９例ＥＬＩＳＡ法检测ＨＢｓＡｇ阴性的消化科住院病人的ＨＢＶ ＤＮＡ，并与１０３例ＥＬＩＳＡ法检测ＨＢｓＡｇ阴性传染科住院肝炎病人相比较。结果：５２．１％的消化科住院病人ＨＢｓＡｇ、抗－ＨＢｓ、抗－ＨＢｅ、抗－Ｈ
|
| 关 键 词： | 乙型肝炎病毒 聚合酶链反应 |
Evaluation of HBV DNA Measured with PCR |
| |
| Zheng Zhen, Li Xin-jian, Yang Shuang-wang, et al. Evaluation of HBV DNA Measured with PCR[J]. Chinese Journal of Clinical Gastroenterology, 1999, 11(3): 116-117 |
| |
| Authors: | Zheng Zhen Li Xin-jian Yang Shuang-wang et al |
| |
| Affiliation: | Zheng Zhen, Li Xin-jian, Yang Shuang-wang, et alDepartment of Gastroenterology, of PLA 454 Hospital |
| |
| Abstract: | Purpose: To evaluated whether the patients with negative HBsAg carry HBV in the depart-ment of gastroenterology of our hospital. Methods: The HBV DNA was measured with polymcrase chain reaction (PCR) technique in 119 patients with negative HBsAg in department of gastroenterology, and compared with 103 patients with negative HBsAg in department of. Results: The results showed that 52. 1% of the patients in the department of gastrogenterology were positive HBVM ( HBsAg, anti-HBs, HBeAg, ani-HBe and anti-HBc), and 30.3% HBV DNAwas positive in the patients. This two indexes were markedly lower compared with the patients in department of. The difference of the rate of positive HBV DNA in the two groups was only in the HBV infective model of negative HBVM. Conclusion: The results suggested that there were HBV carriers in negative HBsAg patients in the department of gastroen-teroloyg, and positive HBVM patients of the two groups actually come from the same group. |
| |
| Keywords: | DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
