Proteomics: analytical tools and techniques |
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Authors: | MacCoss M J Yates J R |
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Institution: | Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92122, USA. maccoss@scripps.edu |
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Abstract: | Scientists have long been interested in measuring the effects of different stimuli on protein expression and metabolism. Analytical methods are being developed for the automated separation, identification, and quantitation of all of the proteins within the cell. Soon, investigators will be able to observe the effects of an experiment on every protein (as opposed to a selected few). This review presents a discussion of recent technological advances in proteomics in addition to exploring current methodological limitations. |
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