前列腺水丸对慢性细菌性前列腺炎大鼠血清IL-6和TNF-α水平的影响

目的:探讨前列腺水丸对慢性细菌性前列腺炎(CBP)大鼠血清白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平的影响。方法:健康成年雄性W istar大鼠50只,体重180~220 g,随机均分为5组:正常对照组、模型组、水丸高剂量组、水丸低剂量组、左氧氟沙星组。前列腺注射大肠埃希菌(107个/m l,0.2 m l/只),建立CBP大鼠动物模型。造模成功1个月后,用前列腺水丸高[32.4 g/(kg.d)]、低[16.2 g/(kg.d)]剂量灌胃治疗,阳性对照组用左氧氟沙星片[0.27 g/(kg.d)]灌胃,模型组和正常对照组用蒸馏水灌胃。连续治疗30 d后,应用放射免疫法检测感染的大鼠血清IL-6、TNF-α。结果:前列腺水丸高、低剂量组大鼠血清IL-6、TNF-α的水平明显低于模型组,与模型组比较差异显著P<0.01)。结论:前列腺水丸对大鼠CBP有一定治疗作用,其作用机制可能与降低血液中细胞因子IL-6、TNF-α水平有关。

Effects of Prostate Water Pellets on the Serum Levels of IL-6 and TNF-α in Rats with Chronic Bacterial Prostatitis

OBJECTIVE: To investigate the effect of Prostate Water Pellets (PWP) on serum levels of IL-6 and TNF-alpha in rats with chronic bacterial prostatitis (CBP). METHODS: Fifty healthy, adult, male Wistar rats with the weight of 180 - 220 g were divided into five groups of ten rats each at random: the control group, model group, high dosage of PWP group, low dosage of PWP group and levofloxacin group. The CBP rat model were created by injecting Escherichia coli (0.2 ml/rat, 10(7)/ml) into prostates. A month later after the model creation, high and low dosage of PWP suspension were used by gavage in CBP rats for 30 days, respectively. Levofloxacin tablets were used by gavage as the positive control, and distilled water was used by gavage in the control and model group. After thirty days, serum levels of IL-6 and TNF-alpha were measured with radioimmunoassay. RESULTS: Compared with the model group, serum levels of IL-6 and TNF-alpha of rats in high and low dosage PWP groups were lower and the difference was significant statistically (P < 0.01). CONCLUSION: It has effect to treat CBP rat with the PWP and its mechanism may relate with the decreasing levels of proinflammatory cytokines(IL-6 and TNF-alpha) in blood.