大鼠动情周期正畸牙移动模型的建立

目的建立生理周期正畸牙移动的实验动物模型。方法选取3个月龄Wistar雌性大鼠200只,随机分为对照组、加力1次对照组和实验组、加力4次对照组和实验组。各组又根据所处动情周期的不同阶段分为4个亚组。加力组分别在动情周期的特定阶段给予重复的间断力1次或4次。每日固定时间对大鼠进行阴道涂片,确定处于动情周期的阶段。实验结束处死大鼠,制备形态学观察标本。结果采用阴道涂片的方法,可以较明确地对大鼠的动情周期进行分期;牙周组织的组织学改变符合正畸牙移动中牙周组织的正常反应,牙周组织处于一种活跃的骨形成和骨吸收的状态。结论本法成功建立了生理周期正畸牙移动的实验动物模型。

Establishment of orthodontic tooth movement model during estrous cycle of rats

Objective To establish an experimental animal model of orthodontic tooth movement during the estrous cycle so as to offer experimental principles for women's orthodontic treatment. Methods 200 female 3-month-old Wistar rats, which had a stable and five-day estrous cycle, were used. They were randomly divided into control group, loading force for one time group and loading force for four times group. Each group was divided again into 4 sub-groups according to the different stage of the estrous cycle. The loading-force groups received repeated intermittent orthodontic force for four times separately during the same stage of the estrous cycle. The vaginal smears were obtained daily to record the duration of the estrous cycle and each stage of a cycle. Then the rats were sacrificed and the specimen were obtained. Results The stage of estrous cycle could be identified by vaginal smears. The histological change of periodontal tissues accorded with the regular reaction during orthodontic tooth movement, which showed an active status of bone resorption and formation in the periodontal tissues. Conclusion The experimental animal model of orthodontic tooth movement during the estrous cycle could be established by the means introduced in this paper.