羟丁酸钠对大鼠离体心脏缺血再灌注损伤的保护作用

对２０只ＳＤ大鼠离体Ｌａｎｇｅｎｄｏｒｆｆ心脏，行全心缺血３０ｍｉｎ再灌注３０ｍｉｎ的方法，研究羟丁酸钠对心肌缺血再灌注损伤的作用和机制。与对照组（ｎ＝１０）相比，缺血前期用含有羟丁酸钠３００ｍｇ／Ｌ的Ｋ－Ｈ液灌注心脏，于再灌注期的冠脉流量（ＣＦ）显著增加，而左室舒张末期压力（ＬＶＥＤＰ）明显下降，同时冠脉流出液中的乳酸脱氢酶（ＬＤＨ）活性和蛋白含量都显著降低；再灌末，心肌组织中超氧化物歧化酶（ＳＯＤ）活性显著高于对照组。提示：羟丁酸钠对离体心脏的缺血再灌注损伤有保护作用，其机制可能与相对提高了心肌组织内的ＳＯＤ活性有关。

PROTECTIVE EFFECTS OF GAMMAHYDROXYBUTYRATE ON MYOCARDIAL ISCHEMIA REPERFUSION INJURY IN THE ISOLATED PERFUSD RAT HEART

The effects of Gammahydroxybutyrate(GHB) on myocardial ischemia reperfusion injury were studied in isolated Langendorff perfused rat heart preparation. Twenty hearts(10 in each gr0up ) were subjected to 30min 0f no-flow global ischemia followed by 30min of reperfusion, As compared with the control hearts,GHB ( 300mg/L) produced significant improvment in recovery of coronary flow when included in the perfusate before ischemia and also attenuated the elevation in left ventricular end-diastolic pressure ( LVEDP) thronghout the reperfusion period. GHB markedly reduced lactate dehydrogenase ( LDH) activity and protein concentration in the coronary effluent. At the end of reperfusion, tissue superoxide dismutase ( SOD) activity was significantly protected from decrease in the hearts pretreated with GHB. The results of this study thus suggest that GHB can reduce ischemia reperfllsion inJury in isolated heart, probably by protecting myocardial tissue SOD activity.