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Blocking effect of serotonin on β-adrenoceptor activity in follicle-enclosed Xenopus oocytes |
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| Authors: | Reiko Fujita Yoshiaki Tamazawa Eric A. Barnard Mitsuhiko Matsumoto |
| Affiliation: | 1 Department of Physiology, School of Medicine, Iwate Medical University, Morioka 020, Japan a Molecular Neurobiology Unit, MRC Centre, Hills Road, Cambridge CB2 2QH, UK |
| Abstract: | The effects of serotonin (5-HT) on a β-adrenergic response were studied, using the voltage clamp technique, in Xenopus laevis oocytes surrounded by their follicular cells. noradrenaline induced marked hyperpolarization, with a specific increase in the permeability of the membrane toward K+. Application of 10 μM 5-HT had little effect on the resting membrane, but significantly depressed the response to 0.1 μM noradrenaline. The dose-response curve for noradrenaline showed a parallel shift to the right in the presence of 5-HT, suggesting that 5-HT competes with noradrenaline for common binding sites at the β-adrenoceptor. |
| Keywords: | β-Adrenoceptors noradrenaline 5-HT (5-hydroxytryptamine, serotonin) Competitive inhibition |
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