| 补阳还五汤对大鼠脑皮层神经元生长的影响 |
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| 引用本文： | 佟丽,曲宏达,陈育尧,沈剑刚. 补阳还五汤对大鼠脑皮层神经元生长的影响[J]. 南方医科大学学报, 2002, 22(8): 711-712 |
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| 作者姓名： | 佟丽 曲宏达 陈育尧 沈剑刚 |
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| 作者单位： | 第一军医大学中医系,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金(39970900)，广东省中医药管理局资助课题(100006) |
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| 摘 要： | 目的观察含补阳还五汤的药物血清对体外培养大鼠脑皮层神经元生长的影响,为补阳还五汤治疗缺血性脑损伤提供实验依据。方法以体外培养新生SD乳鼠大脑皮层神经元为观察对象,采用四唑盐(MTT)比色法测定细胞生长情况。观察含药血清对常规培养及在缺氧状态下神经元生长的影响。结果补阳还五汤对常规培养及在缺氧条件下培养的神经元有显著促进生长作用,与空白血清组比较有显著差(P<0.05)。结论大鼠以补阳还五汤灌胃给药后,其血清中的药物成份有促进体外培养神经元细胞生长的作用。
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| 关 键 词： | 补阳还五汤 血清药理学 神经元 缺氧再给氧损伤 |
| 文章编号： | 1000-2588(2002)08-0711-02 |
| 修稿时间： | 2002-01-25 |
Effects of Buyanghuanwu decoction (BYHWT) on proliferation of cultured rat cortical neurons |
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| TONG Li,QU Hong-da,CHEN Yu-yao,SHEN Jian-gang. Effects of Buyanghuanwu decoction (BYHWT) on proliferation of cultured rat cortical neurons[J]. Journal of Southern Medical University, 2002, 22(8): 711-712 |
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| Authors: | TONG Li QU Hong-da CHEN Yu-yao SHEN Jian-gang |
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| Affiliation: | TONG Li,QU Hong-da,CHEN Yu-yao,SHEN Jian-gang Department of Traditional Chinese Medicine,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To observe the effect of rat serum containing Buyanghuanwu decoction (BYHWT) on the proliferation of cultured rat cortical neurons, so as to understand the mechanism of BYHWT in the treatment of hypoxia brain damage. Methods The growth of cultured rat cortical neurons were observed by MTT assay to evaluate the effect of the serum containing BYHWT on the neurons cultured in both normal and hypoxia conditions. Results BYHWT significantly promoted proliferation of the neurons cultured under both normal and hypoxia conditions, in comparison with the response of the cells to drug-free serum (P<0.05). Conclusion Some of the constituents of BYHWT in rat serum can promote the proliferation of rat cortical neurons cultured in both normal and hypoxia conditions. |
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| Keywords: | BYHWT sera pharmacology cortical neurons hypcxia-reoxyg enation injury |
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