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Increased humoral and cellular immunity in mice inoculated with allogeneic tumor cells attached to microcarrier beads |
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| Authors: | Eitan Yefenof Steven Schwartz Aviva Katz-Gross |
| Affiliation: | Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, P.O.B. 1172 Jerusalem, 91010, Israel |
| Abstract: | A murine carcinoma cell line was grown in a microcarrier culture and was used for immunization of allogeneic mice. It was found that inoculation of cells attached to microcarrier beads resulted in heightened serum titers of cytotoxic antibodies and in a stronger cell-mediated cytotoxic reactivity in the spleen compared to cells detached from the substrate. It is proposed that immunization of animals with anchorage-dependent cells should be carried out while the cells are still adherent to the culture microcarriers. |
| Keywords: | microcarrier beads adherent cell immunization B6 C57BL/6 mice C′ complement Cl cytotoxic index HBSS Hank's balanced salt solution LU lytic unit M+beads M-109 together with beads M/beads M-109 cells attached to beads |
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