Effect of Fc receptor blocking on human natural and lectin-dependent cell-mediated cytotoxicity against adherent HEp-2 cells |
| |
Authors: | András Perl Rhenso Gonzalez-Cabello Éva Pócsik István Láng Péter Gergely |
| |
Affiliation: | Second Department of Medicine, Semmelweis University Medical School, Szentkirályi u. 46, H-1088, Budapest, Hungary |
| |
Abstract: | The effect of Fc receptor (FcR) blocking by aggregated human gamma-globulin (AGG) was studied on natural (NCMC) and lectin-dependent cell-mediated cytotoxicity (LDCC) against adherent HEp-2 human epipharynx carcinoma target cells. Cytotoxicity was measured by detachment from the monolayer of 3H]TdR-prelabelled HEp-2 cells. LDCC was evaluated in a 24 h assay at 50:1 effector-target cell ratio in the presence of 25 μg/ml concanavalin A (Con A). Under these conditions but without Con A considerable NCMC was not elicited by normal lymphocytes. FcR blocking by AGG treatment of effector cells resulted in a significant NCMC activity to HEp-2 targets. In contrast, AGG treatment profoundly depressed LDCC. Monocyte depletion of effector cells had no major influence on the effect of AGG on NCMC and LDCC activities. An interference of FcR blocking by AGG and LDCC in response to Con A is suggested. |
| |
Keywords: | Fc receptor blocking NCMC LDCC |
本文献已被 ScienceDirect 等数据库收录! |