| MALDiI Botyper与API20E对克罗诺杆菌(阪崎肠杆菌)的鉴定结果比较 |
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| 引用本文： | 赵贵明,杨海荣,赵勇胜,袁飞,陈颖.MALDiI Botyper与API20E对克罗诺杆菌(阪崎肠杆菌)的鉴定结果比较[J].中国卫生检验杂志,2010(3). |
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| 作者姓名： | 赵贵明 杨海荣 赵勇胜 袁飞 陈颖 |
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| 作者单位： | 中国检验检疫科学研究院食品安全研究所; |
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| 基金项目： | “十一五”国家科技支撑计划课题(2006BAD04A08); 国家质检总局科技计划项目(2007IK180) |
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| 摘 要： | 目的:评价MALDI-TOF MS Biotyper鉴定克罗诺杆菌的效果。方法:应用MALDI-TOF MS Biotyper与API20E分别对32株克罗诺杆菌(28株分离株,4株参考菌株)与相近菌株阴沟肠杆菌、产气肠杆菌进行鉴定,并对鉴定结果分析比较。结果:MALDI-TOF MS Biotyper2.0将32株克罗诺杆菌鉴定到种、属水平分别为56.2%和37.5%;API20E为75%、21.9%。3株菌未获得鉴定结果,其余29株菌的鉴定结果相符。结论:MALDI-TOF MS Biotyper作为一种新的细菌鉴定手段,可用于克罗诺杆菌的鉴定。
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| 关 键 词： | 克罗诺杆菌 MALDI-TOFMS Biotyper API20E 比较 |
Comparison of MALDI-TOF MS biotyper with the phenotyping methods API20E for identification of Cronobacter spp. |
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| ZHAO Gui-ming,YANG Hai-rong,ZHAO Yong-sheng,YUAN Fei,CHEN Ying.Comparison of MALDI-TOF MS biotyper with the phenotyping methods API20E for identification of Cronobacter spp.[J].Chinses Journal of Health Laboratory Technology,2010(3). |
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| Authors: | ZHAO Gui-ming YANG Hai-rong ZHAO Yong-sheng YUAN Fei CHEN Ying |
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| Institution: | ZHAO Gui-ming,YANG Hai-rong,ZHAO Yong-sheng,YUAN Fei,CHEN Ying(Institute of Food Safety,Chinese Academy of Inspection , Quarantine,Beijing 100123,China) |
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| Abstract: | Objective:To evaluate the identification effect of MALDI-TOF MS Biotyper on Cronobacter spp.Methods:A total of 32 Cronobacter spp.strains(28 isolates,4 reference strains) and similar trains of Enterobacter cloacae,Enterobacter aerogenes were identified with MALDI-TOF MS Biotyper and API20E,respectively.Results:The identification rates of MALDI-TOF MS Biotyper for 32 Cronobacter spp.strains were 56.2% at species level,37.5% at genus level.The identification rates of API20E 32 Cronobacter spp.strains were 75%... |
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| Keywords: | Cronobacter spp MALDI-TOF MS Biotyper API20E Comparison |
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